Characterization of homeodomain-leucine zipper genes in the fern Ceratopteris richardii and the evolution of the homeodomain-leucine zipper gene family in vascular plants
Characterization of homeodomain-leucine zipper genes in the fern Ceratopteris richardii and the evolution of the homeodomain-leucine zipper gene family in vascular plants
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DOI：
10.1093/oxfordjournals.molbev.a026135
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发表时间：
1999-04-01
影响因子：
10.7
通讯作者：
Hasebe, M
中科院分区：
文献类型：
--
作者：
Aso, K;Kato, M;Hasebe, M
同源结构域-亮氨酸拉链(HD-Zip)基因编码转录因子，其特征是同时存在同源结构域和亮氨酸拉链基序。它们属于同源框基因超家族，只在开花植物中被报道过。本文首次报道了从同孢子蕨类植物中分离到的蕨类植物HD-Zip基因(命名为Crhb1-Crhb11)。与已报道的被子植物HD-Zip基因的系统发育分析表明，Ci-Hb基因属于被子植物HD-Zip亚家族中的三个亚家族(HD-Zip I、HD-Zip II和HD-Zip IV)，表明这些I-ID-Zip基因亚家族起源于蕨类植物和种子植物谱系分化之前。Crhb4-Crhb8和Crhb11基因属于HD-Zip I亚家族，但与被子植物KD-Zip I基因不同，亮氨酸拉链基序中含有7个氨基酸。Northern分析表明，Crhb1和Crhb3仅在配子体组织中表达。Crhb2和Crhb11基因在所检测的所有组织中均未检测到表达，而其他CI Hb基因在大多数孢子体和配子体组织中均有表达。虽然Ceratopteris中crhb基因的功能尚不清楚，但它们的表达模式表明，它们调节着蕨类植物配子体和孢子体世代共同的发育或生理过程。Crhb1基因在雄配子体和雌雄配子体混合群体中的表达差异表明，Crhb1基因参与了配子体性别决定。
The homeodomain-leucine zipper (HD-Zip) genes encode transcription factors that are characterized by the presence of both a homeodomain and a leucine zipper motif. They belong to the homeobox gene superfamily and have been reported only from flowering plants. This article is the first report on the fern HD-Zip genes (named Crhb1-Crhb11) isolated from the homosporous fern Ceratopteris richardii. Phylogenetic analyses of the ii Ci Crhb genes with previously reported angiosperm HD-Zip genes show that the Ci hb genes belong to three of the four different angiosperm HD-Zip subfamilies (HD-Zip I, II, and IV), indicating that these subfamilies of I-ID-Zip genes originated before the diversification of the fern and seed plant lineages. The Crhb4-Crhb8 and Crhb11 genes belongs to the HD-Zip I subfamily but differ from angiosperm KD-Zip I genes by the presence of a seven-amino-acid indel in the leucine zipper motif. By the northern analyses, Crhb1 and Crhb3 were expressed only in gametophyte tissue. Expression of Crhb2 and Crhb11 genes could not be detected in any tissue examined, while all other CI hb genes were expressed in most sporophytic and gametophytic tissues. Although the functions of the Crhb genes in Ceratopteris are unknown, their patterns of expression suggest that they regulate developmental or physiological processes common to both the gametophyte and the sporophyte generations of the fern. Differences in the expression of Crhb1 between male gametophytes and male-hermaphrodite mixed populations of gametophytes suggests that the Crhb1 gene is involved in gametophytic sex determination.