INFLUENCE OF EXOGENOUS HORMONES ON SOMATIC EMBRYOGENESIS IN HEVEA-BRASILIENSIS
INFLUENCE OF EXOGENOUS HORMONES ON SOMATIC EMBRYOGENESIS IN HEVEA-BRASILIENSIS
复制标题
DOI：
10.1093/oxfordjournals.aob.a088191
复制
发表时间：
1991-06-01
期刊：
影响因子：
4.2
通讯作者：
DAUZAC, J
中科院分区：
文献类型：
--
作者：
ELHADRAMI, I;CARRON, MP;DAUZAC, J
从种子的内皮层生成体细胞胚胎是很困难的。与其他木质植物一样，橡胶树通常被认为在体细胞胚胎发生方面具有顽抗性。在本文中，我们表明，当3, 4-二氯苯氧基乙酸（3, 4-D）和苄氨基嘌呤（BAP）的常规用量减少2-4倍，并且暴露于这些激素的时间也减少时，愈伤组织的胚胎发生能力增加，而不影响愈伤组织发生。获得了几个胚和小植株的萌发。我们得出的结论是，培养基中过量的生长素和细胞分裂素，即使是弱生长素，如 3、4-D，以及长时间暴露于这些激素，也会抑制愈伤组织的胚胎发生能力。
Generating somatic embryos from the inner teguments ofHeveaseeds is difficult. Like other ligneous plants, the rubber-tree is generally considered to be recalcitrant with regard to somatic embryogenesis. In this paper we show that when conventionally used amounts of both 3, 4-dichlorophenoxyacetic acid (3, 4-D) and benzylaminopurine (BAP) are reduced by 2–4 times and when the length of exposure to these hormones is also reduced, the embryogenic capacity of calli increases without affecting callogenesis. The germination of several embryos and plantlets was obtained. We conclude that an excess of auxin and cytokinin in the medium, even of a weak auxin such as 3, 4-D, and the prolonged exposure to these hormones suppress the embryogenic capacity of the callus.