YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus.
YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus.
复制标题
DOI：
10.1038/s41598-017-09124-1
复制
发表时间：
2017-08-17
影响因子：
4.6
通讯作者：
Hebert EM
中科院分区：
文献类型：
--
作者：
Brown L;Villegas JM;Elean M;Fadda S;Mozzi F;Saavedra L;Hebert EM
乳酸菌的蛋白质分解系统在细菌生长中起着至关重要的作用，有助于发酵产品的风味形成，并能在牛奶发酵过程中释放出具有生物活性的有益健康的多肽。在这项工作中，对德氏乳杆菌亚种蛋白分解系统中涉及的所有基因进行了基因组分析。Lactis CRL-581检查。鉴定了编码细胞膜相关蛋白水解酶、两个多肽转运系统和16个多肽酶的基因。多肽供应对德氏乳杆菌亚种蛋白分解系统相关23个基因转录的影响乳酸细胞在化学限定的培养液(CDM)和CDM+Casitone中生长后进行检测。PrtL、oppa 1、opts、opta基因以及oppDFBC和optBCDF操纵子是CDM中表达最高的基因；它们的表达被多肽抑制6~115倍。蛋白质组学证实了转录分析结果；在无肽的情况下，PrtL、PEPG、OPPD和OptF蛋白表达上调，而DNA结合蛋白YebC在多肽的作用下表达上调。凝胶迁移率改变分析表明，YebC与prtL、oppa 1和opts的启动子区域结合，表明YebC是关键蛋白水解酶基因的转录抑制因子。
The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrueckii subsp. lactis CRL 581 was performed. Genes encoding the cell envelope-associated proteinase, two peptide transport systems, and sixteen peptidases were identified. The influence of the peptide supply on the transcription of 23 genes involved in the proteolytic system of L. delbrueckii subsp. lactis was examined after cell growth in a chemically defined medium (CDM) and CDM supplemented with Casitone. prtL, oppA 1, optS, optA genes as well as oppDFBC and optBCDF operons were the most highly expressed genes in CDM; their expression being repressed 6- to 115-fold by the addition of peptides. The transcriptional analysis was confirmed by proteomics; the up-regulation of the PrtL, PepG, OppD and OptF proteins in the absence of peptides was observed while the DNA-binding protein YebC was up-regulated by peptides. Binding of YebC to the promoter region of prtL, oppA 1, and optS, demonstrated by electrophoretic mobility shift assays, showed that YebC acts as a transcriptional repressor of key proteolytic genes.
登录
查看更多内容
影响因子：
3.3
作者：
Cai H;Thompson R;Budinich MF;Broadbent JR;Steele JL
通讯作者：
Steele JL
影响因子：
4.4
作者：
Hebert, Elvira Maria;Mamone, Gianfranco;Addeo, Francesco
通讯作者：
Addeo, Francesco
影响因子：
4.8
作者：
den Hengst, CD;van Hijum, SAFT;Kuipers, OP
通讯作者：
Kuipers, OP
影响因子：
2.9
作者：
Candiano, G;Bruschi, M;Righetti, PG
通讯作者：
Righetti, PG
影响因子：
3.6
作者：
Guédon, E;Serror, P;Delorme, C
通讯作者：
Delorme, C