SEX-SPECIFIC TRANSCRIPTIONAL REGULATION BY THE MALE AND FEMALE DOUBLESEX PROTEINS OF DROSOPHILA
SEX-SPECIFIC TRANSCRIPTIONAL REGULATION BY THE MALE AND FEMALE DOUBLESEX PROTEINS OF DROSOPHILA
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DOI：
10.1101/gad.7.1.42
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发表时间：
1993-01-01
影响因子：
10.5
通讯作者：
WENSINK, PC
中科院分区：
文献类型：
--
作者：
COSCHIGANO, KT;WENSINK, PC
果蝇的体细胞性别表型受性分化途径的调控。雄性(DsX(M))和雌性(DsX(F))蛋白由该途径末端的双性基因(DsX)编码，结合在127个碱基的增强子的三个位置上，该增强子指导卵黄蛋白基因的性别和组织特异性转录。我们描述了这些结合位点的突变以及由此产生的对体外对dsx(M)和dsx(F)结合的影响，以及对野生型和dsx突变果蝇的基因调控的影响。结果表明，DsX(M)抑制转录，DsX(F)从两个最强结合部位激活转录。因此，该途径通过直接作用于靶基因的雄性和雌性dsx蛋白来调节性特异转录，但具有相反的效果。
The somatic sexual phenotype of Drosophila is regulated by the sexual differentiation pathway. Male (DSX(M)) and female (DSX(F)) proteins encoded by doublesex (dsx), a gene at the end of this pathway, bind to three sites within a 127-bp enhancer that directs sex- and tissue-specific transcription of Yolk protein genes. We describe mutagenesis of these binding sites and the resulting effects on DSX(M) and DSX(F) binding in vitro and on gene regulation in wild-type and dsx mutant flies. The results demonstrate that DSX(M) represses and DSX(F) activates transcription from the two strongest binding sites. Thus, the pathway regulates sex-specific transcription through the male and female dsx proteins that act directly on the target gene, but with opposite effects.