RNA interference in adult Ascaris suum--an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite.
RNA interference in adult Ascaris suum--an opportunity for the development of a functional genomics platform that supports organism-, tissue- and cell-based biology in a nematode parasite.
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DOI：
10.1016/j.ijpara.2015.05.003
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发表时间：
2015-09
影响因子：
4
通讯作者：
Mousley A
中科院分区：
文献类型：
--
作者：
McCoy CJ;Warnock ND;Atkinson LE;Atcheson E;Martin RJ;Robertson AP;Maule AG;Marks NJ;Mousley A
RNA干扰(RNAi)在猪蛔虫成虫体内很容易实现。RNAi能够在成虫体内传播。多个猪链霉菌组织特异性靶点对RNAi易感。双链RNA鸡尾酒有效地同时沉默多个目标。在猪链霉菌的地理分离株中，RNAi是一致的。动物寄生线虫的可持续控制需要开发高效的功能基因组学平台，以促进靶标验证和加强驱虫药的发现。不幸的是，RNA干扰(RNAi)用于验证线虫新药物靶点的有效性仍然存在问题。猪蛔虫是一种重要的兽医寄生虫，也是人畜共患病的病原体。在这里，我们展示了成体猪链霉菌具有RNAi能力，并强调了RNAi在多种组织类型中的诱导、传播和一致性。这一平台为开展整体、组织和细胞水平的基因功能研究提供了新的机会，以加强具有兽医/医学意义的线虫寄生虫的靶标验证过程。
RNA interference (RNAi) is readily achievable in adult Ascaris suum. RNAi is capable of spreading in adult A. suum. Multiple A. suum tissue-specific targets are susceptible to RNAi. Double-stranded RNA cocktails efficiently silence multiple targets simultaneously. RNAi is consistent across geographical isolates of A. suum. The sustainable control of animal parasitic nematodes requires the development of efficient functional genomics platforms to facilitate target validation and enhance anthelmintic discovery. Unfortunately, the utility of RNA interference (RNAi) for the validation of novel drug targets in nematode parasites remains problematic. Ascaris suum is an important veterinary parasite and a zoonotic pathogen. Here we show that adult A. suum is RNAi competent, and highlight the induction, spread and consistency of RNAi across multiple tissue types. This platform provides a new opportunity to undertake whole organism-, tissue- and cell-level gene function studies to enhance target validation processes for nematode parasites of veterinary/medical significance.