Acetylation of poly(amidoamine) dendrimers
Acetylation of poly(amidoamine) dendrimers
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DOI：
10.1021/ma021540e
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发表时间：
2003-07-29
期刊：
影响因子：
5.5
通讯作者：
Baker, JR
中科院分区：
文献类型：
--
作者：
Majoros, IJ;Keszler, B;Baker, JR
采用电位滴定法、凝胶渗透色谱法和核磁共振波谱法验证了聚氨基胺（PAMAM）树状大分子第5代（G5）表面胺的乙酰化所需的精确化学计量学。采用电位滴定法和GPC法测定G5 PAMAM的平均伯胺基数、绝对分子量和分子量分布。这些基本参数用于设计乙酰化反应的化学计量学，该反应产生了大分子上从0到100%的伯胺的乙酰化分数。GPC折射率检测器证实树状大分子的直径与乙酰化程度成反比。乙酰化的树状大分子不遵循传统聚合物分子的洗脱行为，这很可能是因为它们的球形和聚阳离子性质。这项研究阐明了乙酰化反应的本质，并提供了一个定义明确的酰基化大分子，它可以作为复杂树突结构发展的支架。
The precise stoichiometry required for the acetylation of surface amines of a poly(amidoamine) (PAMAM) dendrimer generation 5 (G5) was verified by using potentiometric titration, gel permeation chromatography, and nuclear magnetic resonance spectroscopy. The average number of primary amine groups, absolute molecular weight, and molecular weight distribution of G5 PAMAM were determined by potentiometric titration and GPC. These fundamental parameters were used to design the stoichiometry of an acetylation reaction that yielded acetylation fractions from 0 to 100% of the primary amines on the macromolecule. GPC refractive index detector confirmed that the diameter of the dendrimer related inversely to the degree of acetylation. The acetylated dendrimers do not follow the elution behavior of the conventional polymer molecules most probably because of their spherical shape and polycationic nature. This study clarifies the nature of the acetylation reaction and provides a well-defined acylated macromolecule, which can serve as a scaffold for the development of complex dendrimeric structures.