Assembly of trunk and limb blood vessels involves extensive migration and vasculogenesis of somite-derived angioblasts
Assembly of trunk and limb blood vessels involves extensive migration and vasculogenesis of somite-derived angioblasts
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DOI：
10.1006/dbio.2001.0267
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发表时间：
2001-06-15
影响因子：
2.7
通讯作者：
Bautch, VL
中科院分区：
文献类型：
--
作者：
Ambler, CA;Nowicki, JL;Bautch, VL
血管发育需要前体细胞组装成血管，但胚胎血管是如何组装的还不清楚。为了确定血管细胞如何迁移并组装成躯干和肢体的血管，在发育中的胚胎中追踪了标记的体节来源的成血管细胞。注射鸟类体节与细胞跟踪器DiI显示，体节衍生的成血管细胞在未受干扰的胚胎迁移广泛，并有助于躯干和Imb血管。与小鼠前体中胚层移植物的小鼠-禽类嵌合体在离移植物显著距离的血管中具有移植物衍生的内皮细胞，表明小鼠成血管细胞在禽类宿主中广泛迁移。小鼠移植物来源的内皮细胞一致地发现在主干血管，如神经周围血管丛，主静脉，和推定的体间血管，以及在血管的肢体和肾脏的雏形。移植物定植的这种可重复的模式表明，鸟类的躯干和四肢血管的血管图案线索被哺乳动物的成血管细胞所识别。用鹌鹑血管标记物QH 1和小鼠血管标记物PECAM-1染色的小鼠-鹌鹑嵌合体具有精细的嵌合血管，移植物衍生的小鼠细胞与移植物细胞定殖的肥大血管床中的鹌鹑血管细胞交错。因此，不同的躯干和四肢血管的内皮细胞，从迁移semitic成血管细胞，这些血管的组装可能有一个大的血管生成成分。(C)北京：科学出版社.
Vascular development requires the assembly of precursor cells into blood vessels, but how embryonic vessels are assembled is not well understood. To determine how vascular cells migrate and assemble into vessels of the trunk and limb, marked somite-derived angioblasts were followed in developing embryos. Injection of avian somites with the cell-tracker DiI showed that somite-derived angioblasts in unperturbed embryos migrated extensively and contributed to trunk and Imb vessels. Mouse-avian chimeras with mouse presomitic mesoderm grafts had graft-derived endothelial cells in blood vessels at significant distances from the graft, indicating that mouse angioblasts migrated extensively in avian hosts. Mouse graft-derived endothelial cells were consistently found in trunk vessels, such as the perineural vascular plexus, the cardinal vein, and presumptive intersomitic vessels, as well as in vessels of the limb and kidney rudiment. This reproducible pattern of graft colonization suggests that avian vascular patterning cues for trunk and limb vessels are recognized by mammalian semitic angioblasts. Mouse-quail chimeras stained with both the quail vascular marker QH1 and the mouse vascular marker PECAM-1 had finely chimeric vessels, with graft-derived mouse cells interdigitated with quail vascular cells in mast vascular beds colonized by graft cells. Thus, diverse trunk and limb blood vessels have endothelial cells that developed from migratory semitic angioblasts, and assembly of these vessels is likely to have a large vasculogenic component. (C) 2001 Academic Press.