THE SACCHAROMYCES-CEREVISIAE CKS1 GENE, A HOMOLOG OF THE SCHIZOSACCHAROMYCES-POMBE SUC1+ GENE, ENCODES A SUBUNIT OF THE CDC28 PROTEIN-KINASE COMPLEX
THE SACCHAROMYCES-CEREVISIAE CKS1 GENE, A HOMOLOG OF THE SCHIZOSACCHAROMYCES-POMBE SUC1+ GENE, ENCODES A SUBUNIT OF THE CDC28 PROTEIN-KINASE COMPLEX
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DOI：
10.1128/mcb.9.5.2034
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发表时间：
1989-05-01
影响因子：
5.3
通讯作者：
REED, SI
中科院分区：
文献类型：
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作者：
HADWIGER, JA;WITTENBERG, C;REED, SI
酿酒酵母基因 CDC28 编码细胞周期启动所需的蛋白激酶。为了鉴定编码与 Cdc28 蛋白激酶相互作用的蛋白质的基因，分离了温度敏感的 cdc28 突变的高拷贝质粒抑制子。其中一个抑制因子 CKS1 被发现编码一种 18 千道尔顿的蛋白质，该蛋白质与裂殖酵母的 scu1+ 蛋白质 (p13) 具有高度同源性（67% 氨基酸序列同一性）。染色体 CKS1 基因的破坏赋予与 cdc28 突变体类似的 G1 停滞表型。使用 Cks-1 特异性抗血清证明了酵母裂解物中存在 18 千道尔顿 Cks1 蛋白。此外，Cks1 蛋白被证明与 Cdc28 蛋白激酶的活性形式有物理相关性。这些数据表明 Cks1 是 Cdc28 蛋白激酶复合物的重要组成部分。
The Saccharomyces cerevisiae gene CDC28 encodes a protein kinase required for cell cycle initiation. In an attempt to identify genes encoding proteins that interact with the Cdc28 protein kinase, high-copy plasmid suppressors of a temperature-sensitive cdc28 mutation were isolated. One such suppressor, CKS1, was found to encode an 18-kilodalton protein that shared a high degree of homology with the scu1+ protein (p13) of Schizosaccharomyces pome (67% amino acid sequence identity). Disruption of the chromosomal CKS1 gene conferred a G1 arrest phenotype similar to that of cdc28 mutants. The presence of the 18-kilodalton Cks1 protein in yeast lysates was demonstrated by using Cks-1 specific antiserum. Furthermore, the Cks1 protein was shown to be physically associated with active forms of the Cdc28 protein kinase. These data suggest that Cks1 is an essential component of the Cdc28 protein kinase complex.