LEISHMANIA-DONOVANI METACYCLIC PROMASTIGOTES - TRANSFORMATION INVITRO, LECTIN AGGLUTINATION, COMPLEMENT RESISTANCE, AND INFECTIVITY
LEISHMANIA-DONOVANI METACYCLIC PROMASTIGOTES - TRANSFORMATION INVITRO, LECTIN AGGLUTINATION, COMPLEMENT RESISTANCE, AND INFECTIVITY
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DOI：
10.1016/0014-4894(87)90138-x
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发表时间：
1987-10-01
影响因子：
2.1
通讯作者：
MILES, MA
中科院分区：
文献类型：
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作者：
HOWARD, MK;SAYERS, G;MILES, MA
用仓鼠脾中的杜氏利什曼原虫无鞭毛体在改良的Grace培养基中建立高密度的无菌培养物，当培养物进料时仅部分补充培养基。小，自由游泳，高度活跃的静止期前鞭毛体与短细胞体和长鞭毛在此培养基中诱导。如此诱导的新鲜转化的静止期前鞭毛体不太能够结合花生凝集素，具有超过40倍的抗正常人血清的杀伤能力，并且与新鲜转化的对数期或长期培养前鞭毛体相比，在体内和体外的感染性增加了15倍。这些短型前鞭毛体可能对应于白蛉媒介中的后环前鞭毛体形式。
Freshly transformed Leishmania donovani amastigotes from hamster spleen were used to establish axenic cultures at high density in a modified Grace''s medium, which was only partly replenished when cultures were fed. Small, free-swimming, highly active stationary phase promastigotes with a short cell body and long flagellum were induced in this medium. The freshly transformed stationary phase promastigotes so induced were less able to bind peanut agglutinin, had more than 40-fold increased resistance to killing by normal human serum, and 15-fold increased infectively both in vivo and in vitro when compared to freshly transformed logarithmic phase or long term culture promastigotes. These short form promastigotes may correspond to the metacyclic promastigote forms in the sand fly vector.