Determining lectin specificity from glycan array data using motif segregation and GlycoSearch software.
Determining lectin specificity from glycan array data using motif segregation and GlycoSearch software.
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DOI：
10.1002/9780470559277.ch130028
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发表时间：
2013
影响因子：
--
通讯作者：
Haab, Brian
中科院分区：
文献类型：
--
作者：
Kletter, Doron;Cao, Zheng;Bern, Marshall;Haab, Brian
The glycan array is a powerful tool for investigating the specificities of glycan-binding proteins. By incubating a glycan-binding protein on a glycan array, the relative binding to hundreds of different oligosaccharides can be quantified in parallel. Based on these data, much information can be obtained about the preference of a glycan-binding protein for specific subcomponents of oligosaccharides, or motifs. In many cases the analysis and interpretation of glycan array data can be time consuming and imprecise if done manually. Recently we developed software, called GlycoSearch, to facilitate the analysis and interpretation of glycan array data based on previously developed methods called Motif Segregation and Outlier Motif Analysis. Here we describe the principles behind the method and the use of this new tool for mining glycan array data. The automated, objective, and precise analysis of glycan array data should enhance the value of the data for a broad range of research applications.
