Intracellular acidification and damage of cellular membrane of Saccharomyces pastorianus by low-pressure carbon dioxide microbubbles
Intracellular acidification and damage of cellular membrane of Saccharomyces pastorianus by low-pressure carbon dioxide microbubbles
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低压二氧化碳微泡对巴氏酵母细胞内酸化及细胞膜损伤
DOI：
10.1016/j.foodcont.2016.07.023
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发表时间：
2017
期刊：
影响因子：
6
通讯作者：
S. Odake
中科院分区：
文献类型：
--
作者：
F. Kobayashi;S. Odake
采用低压二氧化碳微泡两阶段法（两阶段MBCO2）研究了细胞内酸化和细胞膜损伤对巴斯德酵母灭活的影响。细胞内/外 pH (pHin 和 pHex) 和碘化丙啶 (PI) 摄取 S。 对经过两阶段MBCO2处理的巴斯德氏细胞进行荧光分析，并与灭活效率进行比较。 pHex 和 pHinofS。 仅用混合容器在 10°C 的两阶段 MBCO2 处理的巴斯德氏细胞低于未处理的细胞。然而，存活数量和pHinofS。 用含有 1.0 MPa 混合容器和 40 °C 加热线圈的两级 MBCO2 处理的巴斯德氏菌细胞随着暴露时间的延长而减少，并且证实了 PI 摄取率的增加。另外，当混合容器的压力为1.0MPa时，存活数和pHinofS。 通过使用两级 MBCO2 加热线圈将温度提高到 45 和 50 °C，巴斯德氏菌细胞显着减少，并且 PI 吸收率增加。这些结果表明 S 的失活。 两阶段 MBCO2 引起的巴斯德氏菌可能是由于 45 和 50 °C 时细胞膜的损伤以及 40 °C 时 pHinat 的降低而引起的。
The effects of intracellular acidification and damage of cellular membrane on the inactivation ofSaccharomycespastorianusby a two-stage method of low-pressure carbon dioxide microbubbles (two-stage MBCO2) were investigated. Intra/extra cellular pH (pHinand pHex) and propidium iodide (PI) uptake ofS. pastorianuscells treated with the two-stage MBCO2were measured by fluorescence analysis, and were compared with the inactivation efficiency. The pHexand pHinofS. pastorianuscells treated with only mixing vessel at 10 °C of two-stage MBCO2was lower than those that were untreated. However, the surviving number and the pHinofS. pastorianuscells treated with the two-stage MBCO2containing the mixing vessel at 1.0 MPa and the heating coil of 40 °C decreased concomitant with the exposure time, and the increase of the PI uptake ratio was confirmed. In addition, when the pressure of the mixing vessel was 1.0 MPa, the surviving number and the pHinofS. pastorianuscells significantly decreased, and the PI uptake ratio increased by increasing the temperature to 45 and 50 °C with the heating coil of the two-stage MBCO2. These results suggested that the inactivation ofS. pastorianusby two-stage MBCO2might be induced by the damage to the cellular membrane at 45 and 50 °C, and by the lowering of the pHinat 40 °C.