The signal flow and motor response controling chemotaxis of sea urchin sperm
The signal flow and motor response controling chemotaxis of sea urchin sperm
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DOI：
10.1038/ncb915
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发表时间：
2003-02-01
影响因子：
21.3
通讯作者：
Weyand, I
中科院分区：
文献类型：
--
作者：
Kaupp, UB;Solzin, J;Weyand, I
对精子趋化性的信号通路和行为策略知之甚少。我们研究了海胆Arbacia punctulata精子趋化性的细胞事件和运动反应。在这里，我们表明，反应，一种化学引诱肽，启动一个快速和短暂的上升，在环GMP的浓度，随后由一个短暂的流入Ca 2+。单个反应分子的结合引起Ca 2+反应，并且50-100个结合分子使反应饱和。记录单个分子的能力让人想起视杆细胞感光器的单光子敏感性。反应物和环核苷酸都能引起精子游动路径的转弯或短暂翻滚。我们的结论是cGMP介导的Ca 2+浓度的增加诱导精子的化学引诱物的初级电机响应。
The signalling pathway and the behavioural strategy underlying chemotaxis of sperm are poorly understood. We have studied the cellular events and motor responses that mediate chemotaxis of sperm from the sea urchin Arbacia punctulata. Here we show that resact, a chemoattractant peptide, initiates a rapid and transient rise in the concentration of cyclic GMP, followed by a transient influx of Ca2+. The binding of a single resact molecule elicits a Ca2+ response, and 50-100 bound molecules saturate the response. The ability to register single molecules is reminiscent of the single-photon sensitivity of rod photoreceptors. Both resact and cyclic nucleotides cause a turn or brief tumbling in the swimming path of sperm. We conclude that a cGMP-mediated increase in the Ca2+ concentration induces the primary motor response of sperm to the chemoattractant.