Validation of a whole slide imaging system for primary diagnosis in surgical pathology: A community hospital experience.
Validation of a whole slide imaging system for primary diagnosis in surgical pathology: A community hospital experience.
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DOI：
10.4103/2153-3539.145731
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发表时间：
2014-01-01
影响因子：
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通讯作者：
O'Neill, Dennis G
中科院分区：
文献类型：
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作者：
Buck, Thomas P;Dilorio, Rebecca;O'Neill, Dennis G
由美国病理学家学院委托的专家小组推荐了用于外科病理初级诊断的验证全玻片成像（WSI）指南。使用这些验证指南的这样一个系统的实施还没有来自社区医院设置的报道。目的是实现WSI系统，验证每个病理学家使用该系统，并将该系统与常规玻片解释并行运行。六位病理学家每人重新检查了大约300个先前诊断的标本，平均分为玻片和数字图像（扫描频率为*20）。计算基线观察者内部不一致率（玻璃对玻璃），并与原始玻片解释和审查的数字玻片解释之间的不一致率进行比较。洗脱期至少为3个月。验证后，在8个月的时间内，使用传统显微镜（TM）和WSI并行诊断每日病例的子集。TM和WSI不一致率分别为3.3% ~ 13.3%和2.1% ~ 10.1%。病理组间差异无统计学意义。类似的研究也得出了相似的不一致率。在我们的实验室，经过适当的实施和培训，WSI和TM方法之间没有差异。
Guidelines for validating whole slide imaging (WSI) for primary diagnosis in surgical pathology have been recommended by an expert panel commissioned by the College of American Pathologists. The implementation of such a system using these validation guidelines has not been reported from the community hospital setting. The objective was to implement a WSI system, validate each pathologist using the system and run the system in parallel with routine glass slide interpretation. Six pathologists re-reviewed approximately 300 previously diagnosed specimens each, divided equally between glass slides and digital images (scanned at *20). Baseline intraobserver discordance rates (glass to glass) were calculated and compared to discordance rates between the original glass slide interpretation and the reviewed digital slide interpretation. A minimum of 3 months was used as the washout period. After validation, a subset of daily cases was diagnosed in parallel using traditional microscopy (TM) and WSI over an 8-month period. The TM and WSI discordance rates ranged from 3.3% to 13.3% and 2.1% to 10.1%, respectively. There was no statistically significant difference among the pathologists. The parallel study yielded similar rates of discordances. In our laboratory, after appropriate implementation and training, there was no difference between the WSI and TM methods.