Auxotrophic Salmonella typhi as live vaccine.
Auxotrophic Salmonella typhi as live vaccine.
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DOI：
10.1016/s0264-410x(88)80017-3
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发表时间：
1988-04
期刊：
影响因子：
5.5
通讯作者：
B. Stocker
中科院分区：
文献类型：
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作者：
B. Stocker
伤寒沙门氏菌541 Ty缺失ataroA和purA，导致需要芳香族代谢物（包括对氨基苯甲酸酯）和腺嘌呤。36名志愿者饮用108 ~ 1010个菌的541 Ty或其Vi阴性突变体543 Ty后，均未出现任何不良反应，均显示出细胞免疫应答，但只有少数血清滴度升高。鼠伤寒实验（在Wellcome研究实验室和斯坦福大学）表明，腺嘌呤的需要可以降低小鼠组织中细菌的存活率和活疫苗的效力。仅通过阻断芳香族生物合成减毒的伤寒沙门氏菌作为口服途径活疫苗可能更有效。
Salmonella typhi541Ty has deletions ataroAandpurA, causing requirement for aromatic metabolites (includingp-aminobenzoate) and for adenine. None of 36 volunteers who drank 108to 1010bacteria of 541Ty or its Vi-negative mutant 543Ty showed any adverse effect; all gave evidence of cellular immune response but only a few had serum titre increases.S. typhimuriumexperiments (at the Wellcome Research Laboratories and at Stanford) show that adenine requirement may reduce both bacterial survival in mouse tissues and live-vaccine efficacy.S. typhiattenuated only by block(s) in aromatic biosynthesis may be more effective as oral-route live vaccine.