Tooth loss, cognitive impairment and chronic cerebral ischemia
Tooth loss, cognitive impairment and chronic cerebral ischemia
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牙齿脱落、认知障碍和慢性脑缺血
DOI：
10.1016/j.jds.2019.09.001
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发表时间：
2020-03-01
影响因子：
3.5
通讯作者：
Jiang, Qingsong
中科院分区：
文献类型：
--
作者：
Pang, Qian;Wu, Qianqian;Jiang, Qingsong
背景/目的：血管因素是认知功能障碍或痴呆发生过程中的重要危险因素。牙齿缺失会导致小鼠的空间学习和记忆障碍，一氧化氮(NO)及其合酶可能参与了这一过程。本研究的目的是观察和比较Wistar大鼠牙齿缺失和慢性脑缺血大鼠的行为障碍，以及在这两种情况下一氧化氮(NO)及其合酶的变化。材料和方法：采用Morris水迷宫测试Wistar大鼠拔除磨牙和阻断2条血管造成脑缺血后8周的空间学习记忆能力。用Griess法、Western blotting和免疫组织化学方法检测NO及其合酶的变化。结果：Wistar大鼠在牙齿缺失和脑缺血后，在空间学习记忆方面也出现了类似的损害。大鼠海马区NO和iNOS水平升高，eNOS水平下降。结论：对于Wistar大鼠，与牙齿缺失相关的认知损害和慢性脑缺血引起的认知损害的结果在统计学上没有显著差异，这两种情况下都涉及到海马区的NO、iNOS和eNOS。(C)2019年中国共和国牙科学会。Elsevier B.V.出版服务。
Background/purpose: Vascular factor is an important risk factor in the process of cognitive impairment or dementia. Tooth loss could cause impairments of spatial learning and memory in mice, and nitric oxide (NO) and its synthase might be involved in the process. The objectives of this study were to investigate and compare the behavioral impairments between the Wistar rats with tooth loss and those with chronic ischemia and to determine the changes in nitric oxide (NO) and its synthases under those two conditions.Materials and methods: The Morris water maze was used to test the spatial learning and memory abilities in the Wistar rats 8 weeks after the molar extraction procedure and the occlusion of 2 blood vessels to produce cerebral ischemia. The changes in NO and its synthases were evaluated using the Griess assay, Western blotting, and immunohistochemistry.Results: Similar impairments in the spatial learning and memory of Wistar rats were found after tooth loss and the induction of cerebral ischemia. The levels of NO and iNOS in the rat hippocampus increased, and the levels of eNOS decreased. Conclusion: For Wistar rats, the results of cognitive impairments related to tooth loss and those that occur due to chronic cerebral ischemia were statistically not significant and that NO, iNOS and eNOS in the hippocampus are involved in both cases. (C) 2019 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.