Pulmonary microcirculatory kinetics of neutrophils deficient in leukocyte adhesion-promoting glycoproteins.
Pulmonary microcirculatory kinetics of neutrophils deficient in leukocyte adhesion-promoting glycoproteins.
复制标题
缺乏白细胞粘附促进糖蛋白的中性粒细胞的肺微循环动力学。
DOI：
10.1152/jappl.1990.69.1.207
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
WagnerJr,WW
中科院分区：
文献类型：
--
作者：
Yoder,MC;Checkley,LL;Giger,U;Hanson,WL;Kirk,KR;Capen,RL;WagnerJr,WW
导致中性粒细胞在肺循环中隔离的机制尚不清楚。由于中性粒细胞表面膜上的CD 11/CD 18糖蛋白家族参与了许多与内皮细胞的粘附相互作用，我们研究了这些蛋白在肺中性粒细胞血管内隔离中的作用。从正常犬和已知患有白细胞粘附缺陷病（CD 11/CD 18粘附家族的遗传性缺陷）的唯一存活犬中分离中性粒细胞。中性粒细胞用荧光素染料标记，注射到正常受体犬中，并通过透明胸窗通过体内视频荧光显微镜记录其通过肺微循环的通道。正常和缺乏中性粒细胞的通过时间在广泛的血液动力学条件下相似。酵母多糖激活的血浆，这增加了表面膜表达的CD 11/CD 18，延长正常中性粒细胞的过境，但没有改变的缺陷中性粒细胞的过境时间。这些结果表明，中性粒细胞CD 11/CD 18粘附促进糖蛋白不参与中性粒细胞的正常肺隔离，但有一个显着的作用，在肺毛细血管中激活的中性粒细胞的逮捕。
The mechanism that causes neutrophils to sequester in the pulmonary circulation is unknown. Because the CD11/CD18 glycoprotein family on the surface membrane of neutrophils participates in many adhesive interactions with the endothelium, we investigated the role of these proteins in the intravascular sequestration of pulmonary neutrophils. Neutrophils were isolated from normal dogs and from the only living dog known to have leukocyte adhesion deficiency disease, an inherited deficiency of the CD11/CD18 adhesion family. The neutrophils were labeled with fluorescein dye, injected into normal recipient dogs, and their passage through the pulmonary microcirculation was recorded by in vivo videofluorescence microscopy through a transparent thoracic window. Transit times for normal and deficient neutrophils were similar over a wide range of hemo-dynamic conditions. Activation by zymosan-activated plasma, which increases the surface membrane expression of CD11/CD18, prolonged the transit of normal neutrophils but did not alter the transit time of the deficient neutrophils. These results indicate that neutrophil CD11/CD18 adhesion-promoting glycoproteins are not involved in the normal pulmonary sequestration of neutrophils but have a significant role in the arrest of activated neutrophils in the pulmonary capillaries.