DNA configurations and concentration in shearing flow near a glass surface in a microchannel
DNA configurations and concentration in shearing flow near a glass surface in a microchannel
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DOI：
10.1122/1.1822930
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发表时间：
2005-01-01
影响因子：
3.3
通讯作者：
Larson, RG
中科院分区：
文献类型：
--
作者：
Fang, L;Hu, H;Larson, RG
我们通过落射荧光显微镜来表征微通道中玻璃表面附近剪切流中X噬菌体脱氧核糖核酸（DNA）分子的构型和浓度。我们观察到，在Poiffille流中，在从玻璃表面延伸到DNA分子轮廓长度的约三分之一的区域上，X噬菌体DNA分子的平均拉伸显著低于本体，与Li等人（2004）在稳定扭转剪切流中获得的结果一致。我们还发现，在同一区域中的DNA分子的浓度是显着低于在散装，在一定程度上，随着增加Weissenberg数增加。基于Jendrejack等人（2003，2004）最近的理论工作，提出了一种简化的解释，用于解释玻璃表面附近的DNA分子的行为，该解释基于壁对链内流体动力学相互作用的影响。(C)2005年，美国流变学会。
We characterize the configurations and concentration of X-phage deoxyribose nucleic acid (DNA) molecules in shear flow near a glass surface in a microchannel through epifluorescence microscopy. We observe that in Poiseuille flow, over a region extending from a glass surface up to about one third of the contour length of the DNA molecule, the average stretch of a X-phage DNA molecule is significantly lower than in the bulk, in agreement with results obtained in a steady torsional shear flow by Li et al. (2004). We also find that the concentration of DNA molecules in this same region is notably lower than in the bulk, to a degree that increases with increasing Weissenberg number. A simplified explanation is proposed for the behavior of DNA molecules near the glass surface based on wall influences on hydrodynamic interaction within the chain, motivated by the recent theoretical work of Jendrejack et al. (2003, 2004). (C) 2005 The Society of Rheology.