The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome
The derivation of highly germline-competent embryonic stem cells containing NOD-derived genome
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DOI：
10.2337/diabetes.52.1.205
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发表时间：
2003-01-01
期刊：
影响因子：
7.7
通讯作者：
Gardner, RL
中科院分区：
文献类型：
--
作者：
Brook, FA;Evans, EP;Gardner, RL
如果能够利用胚胎干细胞和同源重组在体内对1型糖尿病(NOD)株的基因进行体内修饰，将对自发的1型糖尿病小鼠模型--非肥胖糖尿病(NOD)株的疾病机制的分析非常有利。然而，具有足够生殖系传播性的NODE ES细胞系还没有报道。我们报道了从NOD和129的F1杂交中获得高生殖系能力的ES细胞系，用于NOD基因打靶。因此，我们开发了来自(Nod X 129)F1 x 129回交1号小鼠的ES细胞系，它们被交叉杂交，以选择已知包含疾病基因座的nod基因组特定区域的纯合性。
It would be extremely advantageous to the analysis of disease mechanisms in the spontaneous mouse model of type 1 diabetes, the nonobese diabetic (NOD) strain, if genes in this strain could be modified in vivo using embryonic stem (ES) cells and homologous recombination. However, a NOD ES cell line: with adequate germline transmission has not yet been reported. We report the development of highly germline-competent ES cell lines from the F1 hybrid of NOD and 129 for use in NOD gene targeting. Consequently, we developed ES cell lines derived from (NOD x 129)F1 x 129 backcross 1 mice, which were intercrossed to select for homozygosity of particular regions of NOD genome known to contain disease loci.