Simple method for estimating trypsin
Simple method for estimating trypsin
复制标题
估算胰蛋白酶的简单方法
作者：
H. Wiggins
在喂食标准试验餐后，十二指肠内容物中胰蛋白酶的估计已被证明对胰腺疾病的诊断提供有用的信息（Lundh, 1962; Cook, Lennard-Jones, Sherif, and Wiggins, 1967）。可用来估计胰蛋白酶的方法要么很复杂（Lundh, 1957），要么需要自动滴定仪（Haverback, Dyce, Gutentag, and Montgomery, 1963）。由于对这种估计的需求相对较少，前者不会在繁忙的常规实验室中得到青睐，并且很难证明自动滴定器的支出是合理的。这里描述的方法操作简单，已被发现适合偶尔使用，只需要普通的实验室设备，并直接以国际单位（当量h +释放/分钟/毫升）给出结果。
The estimation of trypsin in duodenal contents following the feeding of a standard test meal has been shown to give useful information in the diagnosis of pancreatic disease (Lundh, 1962; Cook, Lennard-Jones, Sherif, and Wiggins, 1967). The methods available for estimating trypsin are either complex (Lundh, 1957) or require apH stat automatic titrator (Haverback, Dyce, Gutentag, and Montgomery, 1963). Since the demand for this estimation is relatively infrequent, the former will not find favour in a busy routine laboratory and it is difficult to justify the expenditure for an automatic titrator. The method described here is simple to do, has been found suitable for occasional use, requires only normal laboratory equipment, and gives the results directly in international units (uEq.H+ released/min./ml.).