An evaluation of detection methods for large lariat RNAs
An evaluation of detection methods for large lariat RNAs
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DOI：
10.1261/rna.7124405
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发表时间：
2005-03-01
期刊：
影响因子：
4.5
通讯作者：
Boeke, JD
中科院分区：
文献类型：
--
作者：
Coombes, CE;Boeke, JD
Ty 1元件是位于酿酒酵母基因组内的长末端重复序列（LTR）反转录转座子。多年来，人们已经知道，2 '-5'磷酸二酯酶Dbr 1 p是有效的Ty 1转座所必需的，它使内含子lariats去分支。最近的一份报告提出了一种有趣的可能性，即Ty 1 RNA作为转座中间体形成了一个lactone。我们着手进一步研究所提出的Ty 1淋巴细胞分支点的性质。然而，使用广泛的技术，我们无法找到任何证据，为拟议的lirconium结构。此外，我们证明，在最初的研究中使用的一些技术描述的laps是能够错误地报告laps结构。因此，Dbr 1蛋白在Ty 1逆转录转座中的作用仍然难以捉摸。
Ty1 elements are long terminal repeat (LTR) retrotransposons that reside within the genome of Saccharomyces cerevisiae. It has been known for many years that the 2'-5' phosphodiesterase Dbr1p, which debranches intron lariats, is required for efficient Ty1 transposition. A recent report suggested the intriguing possibility that Ty1 RNA forms a lariat as a transposition intermediate. We set out to further investigate the nature of the proposed Ty1 lariat branchpoint. However, using a wide range of techniques we were unable to find any evidence for the proposed lariat structure. Furthermore, we demonstrate that some of the techniques used in the initial study describing the lariat are capable of incorrectly reporting a lariat structure. Thus, the role of the Dbr1 protein in Ty1 retrotransposition remains elusive.