Regulation of cofilin phosphorylation in glomerular podocytes by testis specific kinase 1 (TESK1).
Regulation of cofilin phosphorylation in glomerular podocytes by testis specific kinase 1 (TESK1).
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DOI：
10.1038/s41598-018-30115-3
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发表时间：
2018-08-16
影响因子：
4.6
通讯作者：
Spurney RF
中科院分区：
文献类型：
--
作者：
Wang L;Buckley AF;Spurney RF
Expression of a constitutively active Rho A (V14Rho) in podocytes in vivo induces albuminuria and foot process (FP) effacement. These effects may be mediated by the Rho A effector Rho kinase (ROK); but inhibition of ROK with Y27632 failed to attenuate albuminuria or FP effacement in V14Rho mice. ROK activates LIM kinases (LIMKs), which phosphorylate and inhibit the actin depolymerizing factor cofilin 1 (CFL1). Sustained phosphorylation of CFL1 is implicated in human nephrotic diseases, but Y27632 did not inhibit phosphorylation of CFL1 in vivo, despite effective ROK inhibition. CFL1 is also phosphorylated by testis-specific kinase 1 (TESK1) on the same serine residue. TESK1 was expressed in podocytes, and, similar to the in vivo situation, Y27632 had little effect on phospho-CFL1 (pCFL1) levels in cultured podocytes. In contrast, Y27632 reduced pCFL1 levels in TESK1 knockout (KO) cells. ROK inhibition enhanced podocyte motility but, the motility promoting effect of Y27632 was absent in TESK1 KO podocytes. Thus, TESK1 regulates podocyte cytoskeletal dynamics in glomerular podocytes and may play an important role in regulating glomerular filtration barrier integrity in glomerular disease processes.
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DOI：
10.1074/jbc.m112.435941
发表时间：
2013-03-22
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Hong L;Kenney SR;Phillips GK;Simpson D;Schroeder CE;Nöth J;Romero E;Swanson S;Waller A;Strouse JJ;Carter M;Chigaev A;Ursu O;Oprea T;Hjelle B;Golden JE;Aubé J;Hudson LG;Buranda T;Sklar LA;Wandinger-Ness A
通讯作者：
Wandinger-Ness A
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
19.6
作者：
Kanda, T;Wakino, S;Saruta, T
通讯作者：
Saruta, T
影响因子：
64.8
作者：
Lappalainen, P;Drubin, DG
通讯作者：
Drubin, DG
DOI：
10.1152/ajprenal.00212.2013
发表时间：
2014-06-15
影响因子：
4.2
作者：
Hall, Gentzon;Rowell, Janelle;Winn, Michelle P.
通讯作者：
Winn, Michelle P.