Depression of contact sensitivity by Pseudomonas aeruginosa-induced suppressor cells which affect the induction phase of immune response
Depression of contact sensitivity by Pseudomonas aeruginosa-induced suppressor cells which affect the induction phase of immune response
复制标题
铜绿假单胞菌诱导的抑制细胞降低接触敏感性，影响免疫反应的诱导期
作者：
C. Garzelli;V. Colizzi;M. Campa;L. Bozzi;G. Falcone
本文研究了绿脓杆菌感染小鼠对恶唑酮接触敏感性降低的细胞学基础。18 h前用恶唑酮致敏的小鼠引流淋巴结细胞在其足垫给药时能够诱导对正常小鼠的接触敏感性。相比之下，当将来自铜绿假单胞菌注射的和恶唑酮致敏的供体的引流淋巴结的细胞注射到正常小鼠的足垫中时，不能诱导接触敏感性，并且当将来自致敏小鼠的淋巴结细胞一起注射到正常受体的足垫中时，能够主动阻断由致敏小鼠的淋巴结细胞引起的免疫过程。铜绿假单胞菌诱导的抑制细胞需要抗原刺激，具有对环磷酰胺敏感的前体，并且不影响接触敏感性的效应机制。因此，结果表明，铜绿假单胞菌通过增强抑制细胞的活性来抑制对恶唑酮的接触敏感性，所述抑制细胞通常在致敏过程中产生，并且可能通过抑制引流淋巴结中T淋巴细胞的正常募集来影响免疫应答的传入支。
The cellular basis of depression of contact sensitivity to oxazolone in mice injected with Pseudomonas aeruginosa was studied. Cells from draining lymph nodes of mice sensitized with oxazolone 18 h previously were able to induce contact sensitivity to normal mice when administered in their footpads. In contrast, cells from draining lymph nodes of P. aeruginosa-injected and oxazolone-sensitized donors failed to induce contact sensitivity when injected in the footpad of normal mice and were capable of actively blocking the immunizing process brought about by lymph node cells from sensitized mice when injected together in the footpad of normal recipients. The P. aeruginosa-induced suppressor cells required antigenic stimulation, had precursors sensitive to cyclophosphamide, and did not affect the effector mechanisms of contact sensitivity. Thus, the results suggest that P. aeurginosa depresses contact sensitivity to oxazolone by enhancing the activity of suppressor cells which normally arise during the sensitization process and which affect the afferent limb of the immune response, probably by inhibiting the normal recruitment of T lymphocytes in the draining lymph nodes.