Effect of hyaluronidase treatment on the distribution of cranial neural crest cells in the chick embryo.
Effect of hyaluronidase treatment on the distribution of cranial neural crest cells in the chick embryo.
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透明质酸酶处理对鸡胚颅神经嵴细胞分布的影响。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
Stephen Meier
中科院分区：
文献类型：
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作者：
Cheryl B. Anderson;Stephen Meier
颅旁轴状中母细胞被划分成称为体粒的节段单位。最近，我们证明了颅神经嵴细胞的迁移途径与鸡胚胎的初代间质之间的形态学关系（Anderson and Meier, '81）。由于细胞外基质，特别是透明质酸，也分布在颅嵴通路中，胚胎在神经管融合和神经嵴迁移之前在囊胚盘下注射透明质酸酶以去除基质。酶处理胚胎的组织学切片显示，透明质酸的阿利新蓝染色明显降低。表面外胚层塌陷，下中胚层也塌陷。胚胎扫描电镜（SEM）显示，尽管由于间充质细胞间的细胞间隙减少，近轴中胚层看起来更浓缩，但仍有节段性图案。在酶处理过的胚胎中，吻嵴细胞像正常情况下一样分布在前四个染色体体的背表面。这种神经嵴细胞的分布即使在酶处理干扰神经管融合时也会发生。我们得出结论：1)神经管融合不是鸡胚胎头嵴及时释放的先决条件；2)在头嵴路径上的许多有组织的富含透明质酸的基质不是头嵴迁移或模式分布所必需的。
The cranial paraxial mesoblast is patterned into segmental units termed somitomeres. Recently we demonstrated the morphological relationship between the migratory pathways of cranial neural crest cells and the patterned primary mesenchyme of chick embryos (Anderson and Meier, '81). Since extracellular matrix, particularly hyaluronate, is also distributed in cranial crest pathways, embryos were given sub-blastodisc injections of hyaluronidase just prior to neural tube fusion and neural crest migration to remove matrix. Histological sections of enzyme-treated embryos showed that Alcian blue staining of hyaluronate was significantly reduced. Surface ectoderm appeared collapsed on the subjacent mesoderm as well. Examination of embryos with the scanning electron microscope (SEM) revealed that paraxial mesoderm remained segmentally patterned even though it appeared more condensed because of a reduction in intercellular space between mesenchymal cells. In enzyme-treated embryos, the rostral crest cells spread over the dorsal surfaces of the first four somitomeres, as they would do normally. This distribution of neural crest cells occurs even when enzyme treatment interferes with neural tube fusion at that level. We conclude that 1) neural tube fusion is not a prerequisite for the timely release of cranial crest in the chick embryo and 2) that much of the organized hyaluronate-rich matrix that lies in the path of cranial crest is not essential for crest emigration or patterned distribution.