A low concentration of azithromycin inhibits the mRNA expression of N-acyl homoserine lactone synthesis enzymes, upstream of lasI or rhlI, in Pseudomonas aeruginosa
A low concentration of azithromycin inhibits the mRNA expression of N-acyl homoserine lactone synthesis enzymes, upstream of lasI or rhlI, in Pseudomonas aeruginosa
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DOI：
10.1016/j.pupt.2009.04.004
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发表时间：
2009-12-01
影响因子：
3.2
通讯作者：
Yamaguchi, Keizo
中科院分区：
文献类型：
--
作者：
Kai, Toshihiro;Tateda, Kazuhiro;Yamaguchi, Keizo
小剂量大环内酯类药物是治疗慢性肺部感染的有效药物，但其作用机制尚不清楚。在这项研究中，我们对低浓度阿奇霉素治疗后的铜绿假单胞菌进行了DNA微阵列分析。我们发现，亚MIC的阿奇霉素不改变群体感应相关基因(LASI、LASR、RHLI、RHLR、VFT、rSAL)的mRNA表达，但降低了LAST和RHLI上游的大多数N-芳基高丝氨酸内酯(AHL)合成酶的表达。我们认为，这些酶的小幅度下调累积导致铜绿假单胞菌AHL产量的较大下降和群体感应的抑制。(C)2009爱思唯尔有限公司。保留所有权利。
Low-dose macrolides are effective therapy in patients with chronic lung infections, but the mechanisms of action are unclear. In this study, we performed DNA microarray analysis of Pseudomonas aeruginosa after treatment with a low concentration of azithromycin. We found that a sub-MIC of azithromycin didn't change mRNA expression of quorum-sensing related genes (lasI, lasR, rhlI, rhlR, vft, rsaL), but lowered expression of most N-aryl homoserine lactone (AHL) synthesis enzymes upstream of last and rhlI. We propose that small down regulation of these enzymes cumulatively resulted in a larger decrease of AHL production and inhibition of quorum-sensing in P. aeruginosa. (c) 2009 Elsevier Ltd. All rights reserved.