DERMAL DEPOSITION OF EOSINOPHIL-GRANULE MAJOR BASIC-PROTEIN IN ATOPIC-DERMATITIS - COMPARISON WITH ONCHOCERCIASIS
DERMAL DEPOSITION OF EOSINOPHIL-GRANULE MAJOR BASIC-PROTEIN IN ATOPIC-DERMATITIS - COMPARISON WITH ONCHOCERCIASIS
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DOI：
10.1056/nejm198508013130502
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发表时间：
1985-01-01
影响因子：
158.5
通讯作者：
GLEICH, GJ
中科院分区：
文献类型：
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作者：
LEIFERMAN, KM;ACKERMAN, SJ;GLEICH, GJ
虽然血液嗜酸性粒细胞增多症是常见的特应性皮炎，组织中的嗜酸性粒细胞的积累并不突出。研究嗜酸性粒细胞脱粒以确定其是否发生在特应性皮炎的病变中。通过免疫荧光法分析组织中嗜酸性粒细胞颗粒主要碱性蛋白的存在。研究了18例特应性皮炎患者的20份活检标本，所有标本均显示嗜酸性粒细胞外的主要碱性蛋白染色。在18例标本中，染色呈纤维状，位于真皮的上半部分，与弹性纤维的分布相似。12例纤维染色标本也显示主要的碱性蛋白染色的细胞外颗粒的形式。一个未受影响的皮肤标本显示出最小的微弱，精细，荧光纤维，但有明显的蛋白质沉积在受影响的皮肤。特应性皮炎中主要碱性蛋白染色的纤维状模式与未经治疗的盘尾丝虫病苔藓样病变非常相似。嗜酸性粒细胞通常在真皮中释放颗粒蛋白，并且不能简单地基于组织中嗜酸性粒细胞的数量来评估嗜酸性粒细胞在疾病中的参与。
Although blood eosinophilia is commonly present in atopic dermatitis, accumulation of tissue eosinophils is not prominent. Eosinophils degranulation was studied to determine if it occurs in lesions of atopic dermatitis. Tissues were analyzed by immunofluorescence for the presence of the eosinophil-granule major basic protein. Twenty biopsy specimens from 18 patients with atopic dermatitis were studied, and all showed major basic protein staining outside eosinophils. In 18 specimens, the staining was fibrillar, was located in the upper half of the dermatis, and was similar to the distribution of elastic fibers. Twelve specimens with fibrillar staining also showed major basic protein staining in the form of extracellular granules. One specimen from unaffected skin showed minimal faint, fine, fluorescing fibrils, but there was marked deposition of the protein in affected skin. The fibrillar pattern of major basic protein staining in atopic dermatis was very similar to that seen in lichenified lesions of untreated onchocerciasis. Eosinophils commonly release granule proteins in the dermis and assessment of eosinophil involvement in disease cannot be based simply on numbers of eosinophils in tissue.