Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates.
Characterization of TEM-1 β-Lactamase-Producing Kingella kingae Clinical Isolates.
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TEM-1 β-内酰胺酶生产 Kingella kingae 临床分离株的表征。
DOI：
10.1128/aac.00318-13
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发表时间：
2013
影响因子：
4.9
通讯作者：
Balashova,NataliyaV
中科院分区：
文献类型：
--
作者：
Banerjee,Anushree;Kaplan,JeffreyB;Soherwardy,Amenah;Nudell,Yoav;Mackenzie,GraceA;Johnson,Shannon;Balashova,NataliyaV
金氏菌是一种人类病原体，可引起儿童和成人的小儿骨关节感染和感染性心内膜炎。该细菌通常对β-内酰胺类抗生素敏感，尽管在罕见的分离株中报告了β-内酰胺类抗生素耐药性。本研究旨在鉴定β-内酰胺类抗生素耐药菌株并分析其耐药机制。筛选一套90 K.从不同的地理位置获得的Kingae临床分离株显示，在从明尼苏达州和冰岛分离的25%的菌株中，对青霉素具有高水平的耐药性。这些菌株产生TEM-1 β-内酰胺酶，并显示含有额外的≥50-kb质粒。对产β-内酰胺酶菌株的染色体外DNA进行Ion Torrent测序，证实了blaTEM基因的质粒定位。多重PCR显示所有β-内酰胺酶产生菌的质粒图谱相同。通过对孔蛋白基因片段的分析，研究了菌株间的亲缘关系。系统发育分析显示，27个单核苷酸多态性（SNPs）的porgene片段，导致两个主要的集群与11个等位基因类型形成细菌菌株亚群。β-内酰胺酶产生者根据基因分型分组。结果提示，产β-内酰胺酶的菌株可能来源于一株携带单一质粒的克雷伯菌。从欧洲传播到美国或从美国传播到欧洲金龟子分离物。这项研究强调了青霉素耐药性的流行K。kingae菌株在某些地区，并强调监测病原体的抗生素耐药性的重要性。
Kingella kingae is a human pathogen that causes pediatric osteoarticular infections and infective endocarditis in children and adults. The bacterium is usually susceptible to β-lactam antibiotics, although β-lactam resistance has been reported in rare isolates. This study was conducted to identify β-lactam-resistant strains and to characterize the resistance mechanism. Screening of a set of 90 K. kingae clinical isolates obtained from different geographic locations revealed high-level resistance to penicillins among 25% of the strains isolated from Minnesota and Iceland. These strains produced TEM-1 β-lactamase and were shown to contain additional ≥50-kb plasmids. Ion Torrent sequencing of extrachromosomal DNA from a β-lactamase-producing strain confirmed the plasmid location of theblaTEMgene. An identical plasmid pattern was demonstrated by multiplex PCR in all β-lactamase producers. The porin gene's fragments were analyzed to investigate the relatedness of bacterial strains. Phylogenetic analysis revealed 27 single-nucleotide polymorphisms (SNPs) in theporgene fragment, resulting in two major clusters with 11 allele types forming bacterial-strain subclusters. β-Lactamase producers were grouped together based onporgenotyping. Our results suggest that the β-lactamase-producing strains likely originate from a single plasmid-bearing K. kingae isolate that traveled from Europe to the United States, or vice versa. This study highlights the prevalence of penicillin resistance among K. kingae strains in some regions and emphasizes the importance of surveillance for antibiotic resistance of the pathogen.