Intranuclear ubiquitin immunoreactivity of the pigmented neurons of the substantia nigra in fatal acute mechanical asphyxiation and drowning
Intranuclear ubiquitin immunoreactivity of the pigmented neurons of the substantia nigra in fatal acute mechanical asphyxiation and drowning
复制标题
致死性急性机械性窒息和溺水时黑质色素神经元的核内泛素免疫反应性
DOI：
10.1007/s004140000200
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发表时间：
2001
影响因子：
2.1
通讯作者：
H. Maeda
中科院分区：
文献类型：
--
作者：
L. Quan;B. Zhu;K. Ishida;S. Oritani;M. Taniguchi;M. Fujita;H. Maeda
为探讨中脑泛素（热休克蛋白）免疫组织化学染色在窒息和溺水法医学诊断中的意义，我们对18例机械性窒息、9例手勒/勒颈、4例上吊、5例误吸/窒息和16例溺水法医学尸检进行了研究。将其与脑干损伤（n = 12）和急性心肌梗死（n = 18）死亡的对照组（n = 30）进行比较。泛素清楚地表明，在黑质色素神经元的细胞核中，显示两种核内染色模式：一种类型的包涵体（可能是Marinesco机构）和弥漫性染色。弥漫性染色在溺水病例中更常见。总的泛素阳性神经元的百分比在绞窄中经常较高（5.1- 28.4%，平均17.0%），吸入/窒息（5.3- 32.0%，平均17.6%）和溺水其中，悬吊（5.1- 12.7%，平均8.6%）、脑干损伤（0 ~ 10.4%，平均5.0%）和急性心肌梗死（1.5- 16.9%，平均8.3%）发生率较低。这些观察结果表明，核内泛素免疫反应性的黑质神经元在中脑引起的窒息和溺水的中枢神经系统的致命的严重压力。
Abstract To evaluate the significance of immunohistochemical staining of ubiquitin (heat shock protein) in the midbrain for the medico-legal diagnosis of fatal asphyxiation and drowning, we investigated forensic autopsy cases of fatal mechanical asphyxia (n = 18), manual/ligature strangulation (n = 9), hanging (n = 4), aspiration/choking (n = 5) and drowning (n = 16). These were compared to control groups (n = 30) consisting of fatalities from brainstem injury (n = 12) and acute myocardial infarction (n = 18). Ubiquitin was clearly demonstrated in the nuclei of pigmented substantia nigra neurons, showing two intranuclear staining patterns: a type of inclusion (possibly Marinesco bodies) and a diffuse staining. The diffuse staining was significantly more frequently observed in cases of drowning. The percentage of total ubiquitin positive neurons was frequently higher in strangulation (5.1– 28.4%, mean 17.0%), aspiration/choking (5.3–32.0%, mean 17.6%) and drowning (7.0–34.1%, mean 19.8%), but relatively low in hanging (5.1–12.7%, mean 8.6%), brainstem injury (0–10.4%, mean 5.0%) and acute myocardial infarction (1.5–16.9%, mean 8.3%). These observations suggest that intranuclear ubiquitin immunoreactivity of the pigmented substantia nigra neurons in the midbrain was induced by a fatal severe stress on the central nervous system in asphyxiation and drowning.
DOI：
10.1056/nejm199911113412011
发表时间：
1999-11
期刊：
The New England journal of medicine
影响因子：
--
作者：
G. Zipfel;J. Lee;D. Choi
通讯作者：
G. Zipfel;J. Lee;D. Choi
应激（热休克）蛋白质在中枢神经系统中积累。
影响因子：
--
作者：
Tytell,M;Barbe,MF;Brown,IR
通讯作者：
Brown,IR