Prior infection and passive transfer of neutralizing antibody prevent replication of severe acute respiratory syndrome coronavirus in the respiratory tract of mice
Prior infection and passive transfer of neutralizing antibody prevent replication of severe acute respiratory syndrome coronavirus in the respiratory tract of mice
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DOI：
10.1128/jvi.78.7.3572-3577.2004
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发表时间：
2004-04-01
影响因子：
5.4
通讯作者：
Murphy, B
中科院分区：
文献类型：
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作者：
Subbarao, K;McAuliffe, J;Murphy, B
鼻内给药后，严重急性呼吸综合征（SARS）冠状病毒在BALB/c小鼠呼吸道中复制到高滴度。在第1天或第2天没有疾病的情况下观察到峰值复制，这取决于给药剂量，病毒在一周内被清除。在病毒复制高峰期，在细支气管上皮细胞中检测到病毒抗原和核酸。小鼠产生了中和抗体反应，并在初次感染后28天免受再感染。被动转移免疫血清给未感染过的小鼠可防止鼻内攻击后病毒在下呼吸道中复制。因此，抗体，单独作用，可以防止SARS冠状病毒在肺中的复制，这是一个有希望的观察，用于疫苗，免疫治疗和免疫预防方案的发展。
Following intranasal administration, the severe acute respiratory syndrome (SARS) coronavirus replicated to high titers in the respiratory tracts of BALB/c mice. Peak replication was seen in the absence of disease on day 1 or 2, depending on the dose administered, and the virus was cleared within a week. Viral antigen and nucleic acid were detected in bronchiolar epithelial cells during peak viral replication. Mice developed a neutralizing antibody response and were protected from reinfection 28 days following primary infection. Passive transfer of immune serum to naive mice prevented virus replication in the lower respiratory tract following intranasal challenge. Thus, antibodies, acting alone, can prevent replication of the SARS coronavirus in the lung, a promising observation for the development of vaccines, immunotherapy, and immunoprophylaxis regimens.