The molecular karyotype of Leishmania major and mapping of alpha and beta tubulin gene families to multiple unlinked chromosomal loci.
The molecular karyotype of Leishmania major and mapping of alpha and beta tubulin gene families to multiple unlinked chromosomal loci.
复制标题
重大利什曼原虫的分子核型以及α和β微管蛋白基因家族到多个未连锁染色体位点的定位。
DOI：
10.1093/nar/13.11.4155
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发表时间：
1985
影响因子：
14.9
通讯作者：
Samaras,N
中科院分区：
文献类型：
--
作者：
Spithill,TW;Samaras,N
采用基因组Southern杂交和脉冲场梯度凝胶电泳（PFG）定位技术，研究了寄生虫硕大利什曼原虫（Leishmaniamorf）微管蛋白基因在染色体上的排列。α-微管蛋白基因以2.4 kb Pst I片段的串联阵列存在，β-微管蛋白基因以3.9 kb Avirin或Pvu I片段的串联阵列存在，但在其他基因组DNA片段上也发现了其他基因。用PFG凝胶电泳法将硕大乳杆菌前鞭毛体释放的染色体大小的DNA分子分离成至少17条染色体带，大小约为400-4000 kb。一些条带可能以非等摩尔量存在，表明可能有超过17条染色体。所有α-微管蛋白基因定位于一条带（7号染色体）。β-微管蛋白基因定位于4条带（第6、10、16和17号染色体）。这表明α-和β-微管蛋白基因家族在L. major中是不连锁的。α-微管蛋白串联阵列存在一个染色体位点，而β-微管蛋白基因既以串联阵列存在，又以分散基因存在于四个染色体位点
The arrangement of tubulin genes in the genome of the protozoan parasite Leishmania major was studied by genomic Southern blot analysis and mapping of genes to chromosomes fractionated by pulsed field gradient gel (PFG) electro-phoresis. α-tubulin genes exist as a tandem array of 2.4 kb PstI fragments, β-tubulin genes are found as a tandem array of 3.9 kb AvaI or PvuI fragments, but additional genes are also found on other genomic DNA fragments. Chromosome-sized DNA molecules released from promastigotes of L.major were fractionated into at least 17 chromosome bands of approximate size 400-4000 kb by PFG gel electrophoresis. Some bands may be present in non-equimolar amounts suggesting that there may be more than 17 chromosomes. All α-tubulin genes were localized to a single band (chromosome 7). β-tubulin genes were localized to four bands (chromosomes 6, 10, 16 and 17). This shows that the α- and β-tubulin gene families are unlinked in L.major. There is a single chromosomal locus for the α-tubulin tandem array whereas β-tubulin genes exist both as a tandem array and as dispersed genes at four chromosomal loci