Interspecific somatic hybridization in Dianthus: selection of hybrids by the use of iodoacetamide inactivation and regeneration ability
Interspecific somatic hybridization in Dianthus: selection of hybrids by the use of iodoacetamide inactivation and regeneration ability
复制标题
石竹种间体细胞杂交：利用碘乙酰胺失活和再生能力选择杂种
DOI：
10.1016/0168-9452(93)90092-e
复制
发表时间：
1993
期刊：
影响因子：
5.2
通讯作者：
M. Mii
中科院分区：
文献类型：
--
作者：
M. Nakano;M. Mii
从石竹叶肉细胞中分离的原生质体。和石竹 (Dianthus caryophyllusL.)由聚乙二醇熔合而成。在融合处理之前，D的原生质体。用碘乙酰胺灭活中华草，抑制细胞分裂。 D.原生质体石竹在本研究中使用的培养条件下分裂形成愈伤组织，但没有观察到芽再生。因此，可以初步选择再生芽的融合来源的愈伤组织作为体细胞杂交细胞系。植物由四种细胞系再生，其中三种在株高和花形态方面表现出亲本的中间特征。酯酶同工酶分析和染色体计数表明它们是种间体细胞杂种植物。这些植物的杂交性质也通过随机扩增多态性 DNA 分析得到证实。
Protoplasts isolated from mesophyll cells ofDianthus chinensisL. andDianthus caryophyllusL. were fused by polyethylene glycol. Prior to fusion treatment, protoplasts ofD. chinensiswere inactivated with iodoacetamide to inhibit cell division. Protoplasts ofD. caryophyllusdivided to form callus under the culture conditions used in this study but no shoot regeneration was observed. Therefore, fusion-derived calli which regenerated shoots could be tentatively selected as somatic hybrid cell lines. Plants were regenerated from four cell lines, and three of them exhibited intermediate characteristics of both parents in plant height and flower morphology. Esterase isozyme analyses and chromosome counts indicated that they were interspecific somatic hybrid plants. The hybrid nature of these plants was also confirmed by random amplified polymorphic DNA analysis.