Different expression of angiogenesis-related factors between human cultivated corneal and oral epithelial sheets
Different expression of angiogenesis-related factors between human cultivated corneal and oral epithelial sheets
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DOI：
10.1016/j.exer.2006.02.015
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发表时间：
2006-10-01
影响因子：
3.4
通讯作者：
Kinoshita, Shigeru
中科院分区：
文献类型：
--
作者：
Sekiyama, Eiichi;Nakamura, Takahiro;Kinoshita, Shigeru
我们开发了一种培养口腔黏膜上皮片（COE）移植系统来治疗严重的人类眼表疾病。与培养的角膜上皮片（CCE）不同，COE诱导轻度浅表外周新生血管形成，尽管中央保持清晰。为了评估CCE和COE在血管生成方面的特征差异，我们检测了CCE和COE中血管生成相关因子的表达。利用CCE和COE样本，我们用免疫组织化学方法测定了血管生成相关因子的蛋白表达：血栓spontin -1 （TSP-1）、色素上皮衍生因子（PEDF）、内皮抑制素、血管抑制素、血管内皮生长因子（VEGF）、fms样酪氨酸激酶I （Flt-1）、激酶插入结构域受体（KDR）和碱性成纤维细胞生长因子（bFGF）。我们使用Western blot分析来确认CCE和COE中免疫组织化学差异的因素。CCE组织中TSP-1的免疫组化染色强度高于COE组织，Western blot分析CCE组织中TSP-1的表达明显高于COE组织（P < 0.05）。PEDF和内皮抑素在CCE中的染色比在COE中的染色稍强。免疫组织化学方面CCE和COE在血管抑制素、VEGF、Flt-1、KDR和bFGF方面无明显差异。与CCE相比，COE降低了抗血管生成因子尤其是TSP-1的表达。这种不同的表达可能与COE移植后遇到的浅表外周新生血管有关。(c) 2006 Elsevier Ltd.版权所有。
We developed a cultivated oral mucosal epithelial sheet (COE) transplantation system to address severe human ocular surface disorders. Unlike the cultivated corneal epithelial sheet (CCE), the COE induces mild superficial peripheral neovascularization although central clarity is maintained. To evaluate the characteristic differences between CCE and COE regarding to angiogenesis, we examined the expression of angiogenesis-related factors in CCE and COE. Using samples of CCE and COE, we immunohistochemically determined protein expression of the angiogenesis related factors: Thrombospondin-1 (TSP-1), pigment epithelium derived factor (PEDF), endostatin, angiostatin, vascular endothelial growth factor (VEGF), Fms-like tyrosine kinase I (Flt-1), kinase insert domain receptor (KDR), and basic fibroblast growth factor (bFGF). We used Western blot analysis to confirm the factors that were immunohistochemically different in CCE and COE. The immunohistochemical staining intensity of TSP-1 was higher in CCE than COE and by Western blot analysis the expression of TSP-1 was significantly higher in CCE than COE (P < 0.05). PEDF and endostatin stained moderately stronger in CCE than COE. Immunohistochemically there was no obvious difference between CCE and COE with respect to angiostatin, VEGF, Flt-1, KDR, and bFGF. In comparison with CCE, COE showed decreased expression of anti-angiogenic factors particularly TSP-1. This different expression may relate to the superficial peripheral neovascularization encountered after COE transplantation. (c) 2006 Elsevier Ltd. All rights reserved.