PHOSPHORUS LIMITATION AND CARBON METABOLISM IN A UNICELLULAR ALGA: INTERACTION BETWEEN GROWTH RATE AND THE MEASUREMENT OF NET AND GROSS PHOTOSYNTHESIS 1
PHOSPHORUS LIMITATION AND CARBON METABOLISM IN A UNICELLULAR ALGA: INTERACTION BETWEEN GROWTH RATE AND THE MEASUREMENT OF NET AND GROSS PHOTOSYNTHESIS 1
复制标题
单细胞藻类中的磷限制和碳代谢：生长速率与净光合作用和总光合作用测量之间的相互作用 1
DOI：
10.1111/j.0022-3646.1983.00185.x
复制
发表时间：
1983
影响因子：
2.9
通讯作者：
B. Piccinin
中科院分区：
文献类型：
--
作者：
G. Harris;B. Piccinin
在一定范围的稀释率下，在磷限制下连续培养莱茵衣原体。使用双同位素（12 C/14 C）技术进行碳吸收测量，并在生长速率的范围内分析了在光照和黑暗中的碳通量。14 C吸收被证明是等于总光合作用只有在最大的相对增长率，在低相对增长率14 C吸收接近净光合作用。发现C吸收的改变模式是由于在低相对生长速率下在光照下暗呼吸的抑制和14 C 02从呼吸途径的释放。14 C从光合途径到呼吸途径的代谢通道发生在低生长速率下，因为呼吸的CO2的比活度达到输入气体混合物的45%。这些数据进行了讨论，在光的总光合作用和净光合作用在自然种群的测量和在光的单细胞藻类的14 C吸收模型的争议。现有的模型被证明是足够的高相对增长率，但不适合低相对增长率下磷限制。
Chlamydomonas reinhardii Dangeard was grown in continuous culture under P limitation at a range of dilution rates. Carbon uptake measurements were performed using double isotope (12C/14C) techniques and the fluxes of carbon in the light and dark were analysed over the range of growth rates. 14C uptake was shown to be equal to gross photosynthesis only at maximum relative growth rates; at low relative growth rates 14C uptake approximated net photosynthesis. The altered pattern of C uptake was found to be due to the suppression of dark respiration in the light and the release of 14C02 from respiratory pathways at low relative growth rates. Metabolic channelling of 14C from photosynthetic pathways to respiratory pathways occurred at low growth rates as the specific activity of the respired CO2 reached 45% of the input gas mixture. These data are discussed in the light of the controversy concerning the measurement of gross and net photosynthesis in natural populations and in the light of models of 14C uptake in single celled algae. Existing models are shown to be adequate for high relative growth rates but not for low relative growth rates under P limitation.