Interleukin-6 stimulates circulating blood-derived endothelial progenitor cell angiogenesis in vitro
Interleukin-6 stimulates circulating blood-derived endothelial progenitor cell angiogenesis in vitro
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DOI：
10.1038/sj.jcbfm.9600509
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发表时间：
2008-01-01
影响因子：
6.3
通讯作者：
Yang, Yuan
中科院分区：
文献类型：
--
作者：
Fan, Yongfeng;Ye, Jianqin;Yang, Yuan
Circulating blood endothelial progenitor cells (EPCs) contribute to postnatal vasculogenesis, providing a novel therapeutic target for vascular diseases. However, the molecular mechanism of EPC-induced vasculogenesis is unknown. Interleukin-6 plays multiple functions in angiogenesis and vascular remodeling. Our previous study demonstrated that the polymorphism (174G > C) in IL-6 gene promoter was associated with brain vascular disease. In this study, we investigated if IL-6 receptor is expressed in human EPCs derived from circulating mononuclear cells, and if interleukin-6 (IL-6) stimulates EPC angiogenesis in vitro. First, we isolated and cultured mononuclear cells from adult human circulating blood. We obtained EPC clones that were further cultured and expended for the angiogenesis study. We found that the EPCs possessed human mature endothelial cell phenotypes; however, they proliferated much faster than mature endothelial cells (P
