Transcription termination factor Pcf11 limits the processivity of Pol II on an HIV provirus to repress gene expression.
Transcription termination factor Pcf11 limits the processivity of Pol II on an HIV provirus to repress gene expression.
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DOI：
10.1101/gad.1542707
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发表时间：
2007-07
影响因子：
10.5
通讯作者：
Zhiqiang Zhang;A. Klatt;A. Henderson;D. Gilmour
中科院分区：
文献类型：
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作者：
Zhiqiang Zhang;A. Klatt;A. Henderson;D. Gilmour
真核生物中的许多延伸因子通过增加RNA聚合酶II(POL II)的加工能力来促进基因的表达。然而，RNA POL II延长复合体的稳定性表明，这种复合体本身不容易过早终止转录，特别是在生理核苷酸浓度下。我们发现终止因子Pcf11会导致HIV前病毒的提前终止。当Pcf11从细胞中耗尽或提取物对6-dichloro-1-beta-D-ribofuranosylbenzimidazole不再敏感时发生的转录，DRB是一种导致提前终止的化合物。因此，在真核细胞中，Pcf11可以作为负延长因子抑制RNAPol II基因的表达。
Many elongation factors in eukaryotes promote gene expression by increasing the processivity of RNA polymerase II (Pol II). However, the stability of RNA Pol II elongation complexes suggests that such complexes are not inherently prone to prematurely terminating transcription, particularly at physiological nucleotide concentrations. We show that the termination factor, Pcf11, causes premature termination on an HIV provirus. The transcription that occurs when Pcf11 is depleted from cells or an extract is no longer sensitive to 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), a compound that causes premature termination. Hence, Pcf11 can act as a negative elongation factor to repress RNA Pol II gene expression in eukaryotic cells.