TRANS-NEURONAL TRANSPORT OF LECTINS
TRANS-NEURONAL TRANSPORT OF LECTINS
复制标题
DOI：
10.1016/0006-8993(85)91187-4
复制
发表时间：
1985-01-01
期刊：
影响因子：
2.9
通讯作者：
COULTER, JD
中科院分区：
文献类型：
--
作者：
FABIAN, RH;COULTER, JD
采用免疫细胞化学染色法对植物凝集素小麦胚芽凝集素（WGA）、油菜凝集素（PSA）、cultinaris凝集素（LCA）、大豆凝集素（SBA）、花生凝集素（PNA）、豆豆蛋白A凝集素（Con A）和欧洲葡萄凝集素（UEA）的轴突和跨神经元转运进行了检测和比较。与n -乙酰氨基葡萄糖和唾液酸碳水化合物残基结合的WGA和3种甘露糖结合凝集素（Con A、PSA和LCA）在注射到面部肌肉后可逆行转运到面神经核，在玻璃体注射后可顺行转运到视神经顶，注射到结膜触须后可顺行转运到三叉神经脊髓尾核。SBA表现出轻微的逆行运输倾向，但没有顺行运输倾向，而UEA和PNA在这些系统中都没有轴向运输。所有顺行运输的凝集素都在各自的末端区标记了神经元体，表明发生了跨神经元运输。凝集素的轴突和跨神经元运输似乎依赖于它们各自的碳水化合物亲和力。某些凝集素的跨神经元转运表明存在神经元交换大分子的机制，这些大分子可能参与介导营养和对靶细胞的其他影响。
Axonal and transneuronal transport of the plant lectins wheat germ agglutinin (WGA), Pisum sativum agglutinin (PSA), Lens cultinaris agglutinin (LCA), soybean agglutinin (SBA), peanut agglutinin (PNA), Concanavalin A agglutinin (Con A), and Ulex europaeus agglutinin (UEA) were examined and compared using an immunocytochemical staining method. WGA, which binds to N-acetylglucosamine and sialic acid carbohydrate residues, and the 3 mannose binding lectins (Con A, PSA and LCA) were found to undergo retrograde transport to the facial nucleus after injection into the facial muscles, and anterograde transport to the optic tectum after injection in the vitreous, and to the spinal trigeminal nucleus caudalis after injection into the mystatial vibrissae. SBA showed a slight tendency to be transported retrogradely, but not in the anterograde direction, whereas UEA and PNA were not axonally transported in any of these systems. All lectins which were transported in the anterograde direction labeled neuronal somata in their respective terminal fields indicating that transneuronal transport had taken place. Axonal and transneuronal transport of the lectins appear to be dependent upon their respective carbohydrate affinities. Transneuronal transport which can be demonstrated for certain lectins indicates that mechanisms exist whereby neurons exchange large molecules which could be involved in mediating trophic and and other influences on target cells.