Odontoblast processes in human dentin revealed by fluorescence labeling and transmission electron microscopy
Odontoblast processes in human dentin revealed by fluorescence labeling and transmission electron microscopy
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DOI：
10.1007/s00418-002-0442-y
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发表时间：
2002-09-01
影响因子：
2.3
通讯作者：
Ozawa, H
中科院分区：
文献类型：
--
作者：
Yoshiba, K;Yoshiba, N;Ozawa, H
在本研究中，成牙本质细胞突起在人牙本质中的分布是通过DiI碳菁染料荧光染色细胞膜来确定的，而F-肌动蛋白是通过罗丹明鬼笔环肽来确定的。共聚焦激光扫描显微镜显示强烈的标记，这两种药物在内部牙本质，而透射电子显微镜（TEM）确定牙本质小管，包括成牙本质细胞的过程中，在这方面，每个过程被包围的细胞膜，并含有丰富的丝状结构。电子致密的“界膜”排列在牙本质小管上。透射电镜下，单个细胞突起向中间区域变窄，其总数也减少。F-肌动蛋白的标记在中间和外部牙本质中都不存在，而DiI的微弱标记沿着牙本质小管直至牙本质-牙釉质交界处（DEJ）可见，在那里它也在小管本身内被识别。透射电镜下，牙本质中、外表面的牙本质小管内实际上是空的，排列着电子致密结构。然而，紧挨着DEJ下方的小管显示出密集的细颗粒物质。因此，我们的研究似乎表明，成牙本质细胞的过程并没有超出内部的牙本质完全爆发的人类前磨牙。
In the present undertaking, the distribution of odontoblast processes in human dentin was determined through the DiI carbocyanine dye fluorescent staining of the cell membrane, while F-actin was identified by rhodamine-phalloidin. Confocal laser scanning microscopy revealed intense labeling for both agents in inner dentin, while transmission electron microscopy (TEM) identified dentinal tubules including odontoblast processes in this area, each process being surrounded by a cell membrane and containing an abundance of filamentous structures. Electron-dense "lamina limitans" lined the dentinal tubules. Individual cell processes became narrower toward the middle area, and their overall numbers decreased as well under TEM. Labeling for F-actin was absent in both middle and outer dentin, while faint labeling for DiI was visible along the dentinal tubules as far as the dentino-enamel junction (DEJ), where it was also recognized within the tubules themselves. Under TEM, the dentinal tubules lined with electron-dense structures were, in fact, empty in the middle and outer dentin. Immediately below the DEJ, however, the tubules manifested dense concentrations of fine granular material. Our study, therefore, appears to suggest that odontoblast processes do not extend beyond the inner dentin of fully erupted human premolars.