Expression of a putative laccase gene, ZmLAC1, in maize primary roots under stress
Expression of a putative laccase gene, ZmLAC1, in maize primary roots under stress
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DOI：
10.1111/j.1365-3040.2005.01435.x
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发表时间：
2006-05-01
影响因子：
7.3
通讯作者：
Wu, YJ
中科院分区：
文献类型：
--
作者：
Liang, MX;Haroldsen, V;Wu, YJ
漆酶是含多个铜离子的糖蛋白，在植物中构成一个多基因家族。然而，它们的生理功能仍未被充分了解。我们从玉米中获得了一个假定的漆酶基因ZmLAC1的序列信息，并详细研究了ZmLAC1的表达。推导的ZmLAC1蛋白与黑麦草的漆酶LpLAC5 - 4有70%的同一性。ZmLAC1在玉米幼苗的叶、茎和根中表达。在未受胁迫的玉米初生根中，与细胞快速分裂和伸长的根尖5毫米相比，在细胞伸长已停止的基部区域ZmLAC - 1转录水平更高。用300毫摩尔/升氯化钠处理导致根伸长区缩短（<2毫米）以及根尖5毫米处肿胀。与形态变化相关的是，ZmLAC1的转录水平在根尖5毫米处提高，达到与基部区域相似的水平。测试的其他非生物胁迫，例如28.5%的聚乙二醇（PEG），其对根伸长的抑制作用与300毫摩尔/升氯化钠相当，但不影响ZmLAC1的转录水平。讨论了ZmLAC1在根对氯化钠或其他高浓度盐响应中的潜在作用。
Laccases are multi-copper-containing glycoproteins and comprise a multi-gene family in plants. However, their physiological functions are still not well understood. We obtained sequence information for a putative laccase gene, ZmLAC1, from maize and studied ZmLAC1 expression in detail. The deduced ZmLAC1 protein was 70% identical to LpLAC5-4, a laccase from ryegrass. ZmLAC1 was expressed in leaves, stems and roots of maize seedlings. In unstressed maize primary roots, a higher ZmLAC-1 transcript level was located in the basal region where cell elongation had ceased compared to the apical 5 mm of the roots where cells were rapidly dividing and elongating. A treatment with 300 mm NaCl resulted in a shortened root elongation zone (< 2 mm) and swelling in the apical 5 mm. Associated with the morphological change, the transcript level of ZmLAC1 was enhanced in the apical 5 mm, reaching a level similar to that in the basal region. Other abiotic stresses tested - such as 28.5% polyethylene glycol (PEG), which caused an inhibition of root elongation comparable to 300 mm NaCl - did not affect ZmLAC1 transcript level. Potential roles of ZmLAC1 in the roots responding to NaCl or other high concentration of salts are discussed.