Pt based enzyme electrode probes assembled with Prussian Blue and conducting polymer nanostructures
Pt based enzyme electrode probes assembled with Prussian Blue and conducting polymer nanostructures
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DOI：
10.1016/j.bios.2004.06.026
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发表时间：
2004-12-15
影响因子：
12.6
通讯作者：
Palleschi, G
中科院分区：
文献类型：
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作者：
Curulli, A;Valentini, F;Palleschi, G
以多孔聚碳酸酯膜为模板，制备了1，2-二氨基苯（1，2-DAB）导电聚合物纳米管，将其置于铂箔上，通过计时库仑法进行电聚合，然后将所得导电聚合物纳米管置于铂电极上，用于负载高分散的普鲁士蓝（PB），作为H2 O2检测的活性组分。PB作为H2 O2催化剂的良好稳定性与复合纳米结构膜中有机非常规导电聚合物的存在有关。用扫描电子显微镜（SEM）和拉曼光谱（Raman）对这些纳米结构聚合物/PB复合膜进行了表征。用循环伏安法测试了非常规导电聚合物纳米管/PB修饰的Pt电极在不同pH值下的稳定性，然后用安培法测试了过氧化氢、抗坏血酸、对乙酰氨基酚、尿酸和乙酰胆碱的活性。葡萄糖氧化酶（GOD）、乳酸氧化酶（LOD）、将L-氨基酸氧化酶（L-AAOD）、醇氧化酶（AOD）、甘油-3-磷酸氧化酶（Glycerol-3-phosphate oxidase，GPO）、赖氨酸氧化酶（LyOx）和胆碱氧化酶（ChOx）固定在Pt电极上的1，2-二氨基苯（1，2-DAB）纳米管支撑的PB层上。进行并使用了不同的酶固定化策略。分析参数，如重现性，干扰抑制，响应时间，存储和传感器的操作稳定性进行了研究和优化。研究结果为设计高灵敏度、稳定性好、无干扰的生物传感器提供了指导。该传感器的检测限为5 × 10 ~（-5）mol·L ~（-1），线性范围为5 × 10 ~（-5）~ 5 × 10 ~（-3）mol·L ~（-1），选择性高，在4 ℃下稳定3个月，在室温下稳定4周。还研究了L-（+）-乳酸、L-亮氨酸、乙醇、甘油-3-磷酸、赖氨酸和胆碱生物传感器的类似分析参数和稳定性。(C)2004 Elsevier B. V.保留所有权利。
Conductive polymer nanotubules of 1,2-diaminobenzene (1,2-DAB) were prepared using a porous polycarbonate membrane template, placed on a Pt foil and used to support the polymer, then, the electropolymerisation was performed by chronocoulometry.The obtained conductive polymer nanostructures were then placed on Pt electrode and used to support highly dispersed prussian blue (PB), which acts as the active component for H2O2 detection. The observed good stability of PB as catalyst of H2O2 was related to the presence of organic non-conventional conducting polymers in a composite nanostructured film. These nanostructured polymer/PB composite films were also characterised by scanning electron microscopy (SEM) and Raman spectroscopy.The non-conventional conducting polymer nanotubules/PB modified Pt electrodes were tested by cyclic voltammeter for stability at different pH values, then, by amperometry, for hydrogen peroxide, ascorbic acid, acetaminophen, uric acid and acetylcholine.Glucose oxidase (GOD), lactate oxidase (LOD), L-aminoacid oxidase (L-AAOD), alcohol oxidase (AOD), glycerol-3-phosphate oxidase (GPO), lysine oxidase (LyOx), and choline oxidase (ChOx) were immobilised on PB layer supported on 1,2-diaminobenzene (1,2-DAB) nanotubules onto the Pt electrodes. Different strategies for enzyme immobilisation were performed and used. Analytical parameters such as reproducibility, interference rejection, response time, storage and operational stability of the sensors have been studied and optimised. Results provide a guide to design high sensitive, stable and interference-free biosensors. The glucose biosensors assembled with nanostructured poly(1,2-DAB) showed a detection limit of 5 x 10(-5) mol l(-1), a wide linearity range (5 x 10(-5) to 5 x 10(-3) mol l(-1)), a high selectivity, a stability of 3 months at 4degreesC, and at least 4 weeks at room temperature. Similar analytical parameters and stability were also studied for L-(+)-lactic acid, L-leucine, ethanol, glycerol-3-phosphate, lysine, and choline biosensors. (C) 2004 Elsevier B.V. All rights reserved.