Glutamine and Glucose as Precursors of Transmitter Amino Acids: Ex Vivo Studies
Glutamine and Glucose as Precursors of Transmitter Amino Acids: Ex Vivo Studies
复制标题
DOI：
10.1111/j.1471-4159.1983.tb08058.x
复制
发表时间：
1983-03
影响因子：
4.7
通讯作者：
H. K. Ward;C. Thanki;H. Bradford
中科院分区：
文献类型：
--
作者：
H. K. Ward;C. Thanki;H. Bradford
摘要：将放射性标记的谷氨酰胺和葡萄糖注入大鼠侧脑室内，以标记体内的递质氨基酸库。靠近侧脑室的脑区（海马、纹状体、下丘脑）比更远的结构（如大脑皮层或小脑）更有效地标记。当考虑到前体特异性放射性的任何差异时，在双细胞实验中，单独或一起给予[U-14 C]葡萄糖、[U-14 C]谷氨酰胺或[3 H]谷氨酰胺，在30 - 150 min内对所有区域进行了几乎相同程度的标记。将体内标记的大脑皮层或海马切片在体外用藜芦碱（75 μM）孵育和刺激;对照培养基中存在河豚毒素（1 μM）。单标记实验表明，[U-14 C]-谷氨酰胺比[U-14 C]葡萄糖更有效地标记可释放的谷氨酸和GABA。双标记实验表明，[3 H]谷氨酰胺和[U-14 C]-葡萄糖在体内一起给药后，标记的谷氨酸和GABA在体外可释放的程度相似。这两种类型的实验empbasise谷氨酰胺在体内的递质谷氨酸和GABA的池作出了很大的贡献。
Abstract: Radiolabelled glutamine and glucose were infused into lateral ventricles of rats in order to label transmitter amino acid pools in vivo. Brain regions close to the lateral ventricle (hippocampus, corpus striatum, hypothalamus) were labelled more effectively than more distant structures such as cerebral cortex or cerebellum. All regions were labelled to much the same extent over 30‐150 min by [U‐14C]glucose, [U‐14C]glutamine, or [3H]glutamine administered alone or together in doublelabel experiments when allowance was made for any differences in precursor specific radioactivities. Slices of cerebral cortex or hippocampus from brains labelled in vivo were incubated and stimulated in vitro with veratrine (75 μM); tetrodotoxin (1 μM) was present in the control medium. Single‐label experiments showed that [U‐14C]‐ glutamine was more effective than [U‐14C]glucose for labelling releasable glutamate and GABA. Double‐label experiments showed that [3H]glutamine and [U‐14C]‐ glucose given together in vivo labelled glutamate and GABA releasable in vitro to a similar extent. Both types of experiment empbasise the large contribution made by glutamine in vivo to pools of transmitter glutamate and GABA.