High prevalence of diverse forms of HIV-1 intersubtype recombinants in Central Myanmar: geographical hot spot of extensive recombination
High prevalence of diverse forms of HIV-1 intersubtype recombinants in Central Myanmar: geographical hot spot of extensive recombination
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DOI：
10.1097/00002030-200309260-00009
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发表时间：
2003-09-26
期刊：
影响因子：
3.8
通讯作者：
Kusagawa, S
中科院分区：
文献类型：
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作者：
Takebe, Y;Motomura, K;Kusagawa, S
目的：为了解引起缅甸中部地区HIV-1流行的分子流行病学和基因结构，探讨该地区HIV流行的成因，于1999-2000年在缅甸中部城市曼德勒的高危人群中进行了HIV-1流行的分子流行病学调查，分析了流行的HIV-1的结构特征。根据gag（p17）和env（C2/V3）区对59份标本进行HIV-1基因分型。测定了亚型不一致的HIV-1分离株的近全长核苷酸序列，并对其重组结构进行了表征。包括CRF01_AE在内的三种HIV-1毒株谱系B'亚型27例（45.8%）（泰国亚型B变异体）C亚型15例（25.4%）（8，13.6%），分布在曼德勒，9例（15.3%）标本在HIV-1基因组不同区域表现出不同的亚型不一致模式。对6株亚型不一致的HIV-1分离株的研究表明，它们是由3种流行亚型的各种组合组成的高度多样的独特重组形式（URF）。一个URF是一个特别复杂的嵌合体，包含三种HIV-1亚型之间的13个重组断点。大约有一半的重组体表现出“假型”病毒体结构，其中包膜糖蛋白的外部部分交换与不同谱系的HIV-1菌株，这表明潜在的选择性优势的“假型”病毒超过亲本strain.Conclusion：这项研究揭示了独特的地理热点在缅甸中部，广泛的重组事件似乎正在不断发生。这反映出存在高度暴露的个人和HIV-1传播的社交网络。(C)2003年利平科特威廉姆斯威尔金斯。
Objectives: To investigate the molecular epidemiology and genetic structure of HIV-1 s causing the epidemic in Central Myanmar and to explore the genesis of HIV epidemic in this area.Design: A molecular epidemiological investigation was conducted in 1999-2000 in the city of Mandalay among high-risk populations and the structural features of circulating HIV-1 s were analyzed.Methods: HIV-1 genotypes of 59 specimens were screened based on gag (pl 7) and env (C2/V3) regions. Near full-length nucleotide sequences of HIV-1 isolates with subtype discordance were determined and their recombinant structures were characterized.Results: Three lineages of HIV-1 strains, including CRF01_AE (27, 45.8%), subtype B' (Thailand variant of subtype B) (15, 25.4%) and subtype C (8, 13.6%), were distributed in Mandalay, while substantial portions (9, 15.3%) of specimens showed various patterns of subtype discordance in different regions of HIV-1 genomes. The study on six HIV-1 isolates with subtype discordance revealed that they were highly diverse types of unique recombinant forms (URFs) comprised of various combinations of three circulating subtypes. One URF was a particularly complex mosaic that contained 13 recombination breakpoints between three HIV-1 subtypes. Approximately half of recombinants showed 'pseudotype' virion structures, in which the external portions of envelope glycoproteins were exchanged with different lineages of HIV-1 strains, suggesting the potential selective advantage of 'pseudotype' viruses over parental strains.Conclusion: The study revealed the unique geographical hot spot in Central Myanmar where extensive recombination events appeared to be taking place continually. This reflects the presence of highly exposed individuals and social networks of HIV-1 transmission. (C) 2003 Lippincott Williams Wilkins.