Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production
Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production
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DOI：
10.1007/s00253-009-1949-0
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发表时间：
2009-08-01
影响因子：
5
通讯作者：
Shimizu, Sakayu
中科院分区：
文献类型：
--
作者：
Kishino, Shigenobu;Ogawa, Jun;Shimizu, Sakayu
植物乳杆菌AKU 1009a有效地将亚油酸转化为顺式-9，反式-11-十八烯二酸（18:2）和反式-9，反式-11-18:2的共轭亚油酸。研究了植物乳杆菌AKU 1009a洗涤细胞对多种多不饱和脂肪酸的转化。除亚油酸外，α -亚麻酸[顺式-9、顺式-12、顺式-15-十八碳四烯酸（18:3）]、-亚麻酸（顺式-6、顺式-9、顺式-12-18:3）、柱二酸（反式-5、顺式-9、顺式-12-18:3）和硬脂酸[顺式-6、顺式-9、顺式-12、顺式-15-十八碳四烯酸（18:4）]也发生转化。菌株转化的脂肪酸具有C18脂肪酸和顺式-9、顺式-12二烯体系的共同结构。从α -亚麻酸中制备了3种主要脂肪酸，分别为顺式-9、反式-11、顺式-15-18:3、反式-9、反式-11、顺式-15-18:3和反式-10、顺式-15-18:2。从γ -亚麻酸中制备了四种主要脂肪酸，分别为顺式-6、顺式-9、反式-11-18:3、顺式-6、反式-11-18:3、顺式-6、反式-10-18:2和反式-10-十八烯酸。菌株将C18脂肪酸的顺式-9、顺式-12二烯体系转化为顺式-9、反式-11和反式-9、反式-11共轭二烯体系。这些共轭二烯被菌株进一步饱和成反式-10单烯体系。该结果为了解厌氧细菌生物加氢途径和建立实际生产共轭脂肪酸的微生物工艺，特别是由α -亚麻酸和γ -亚麻酸生产的共轭脂肪酸提供了有价值的信息。
Lactobacillus plantarum AKU 1009a effectively transforms linoleic acid to conjugated linoleic acids of cis-9,trans-11-octadecadienoic acid (18:2) and trans-9,trans-11-18:2. The transformation of various polyunsaturated fatty acids by washed cells of L. plantarum AKU 1009a was investigated. Besides linoleic acid, alpha-linolenic acid [cis-9,cis-12,cis-15-octadecatrienoic acid (18:3)], gamma-linolenic acid (cis-6,cis-9,cis-12-18:3), columbinic acid (trans-5,cis-9,cis-12-18:3), and stearidonic acid [cis-6,cis-9,cis-12,cis-15-octadecatetraenoic acid (18:4)] were found to be transformed. The fatty acids transformed by the strain had the common structure of a C18 fatty acid with the cis-9,cis-12 diene system. Three major fatty acids were produced from alpha-linolenic acid, which were identified as cis-9,trans-11,cis-15-18:3, trans-9,trans-11,cis-15-18:3, and trans-10,cis-15-18:2. Four major fatty acids were produced from gamma-linolenic acid, which were identified as cis-6,cis-9,trans-11-18:3, cis-6,trans-9,trans-11-18:3, cis-6,trans-10-18:2, and trans-10-octadecenoic acid. The strain transformed the cis-9,cis-12 diene system of C18 fatty acids into conjugated diene systems of cis-9,trans-11 and trans-9,trans-11. These conjugated dienes were further saturated into the trans-10 monoene system by the strain. The results provide valuable information for understanding the pathway of biohydrogenation by anaerobic bacteria and for establishing microbial processes for the practical production of conjugated fatty acids, especially those produced from alpha-linolenic acid and gamma-linolenic acid.