QUALITATIVE-ANALYSIS OF THE EXPRESSION OF EPSTEIN-BARR-VIRUS LYTIC GENES IN NASOPHARYNGEAL CARCINOMA BIOPSIES
QUALITATIVE-ANALYSIS OF THE EXPRESSION OF EPSTEIN-BARR-VIRUS LYTIC GENES IN NASOPHARYNGEAL CARCINOMA BIOPSIES
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DOI：
10.1099/0022-1317-76-6-1401
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发表时间：
1995-06-01
影响因子：
3.8
通讯作者：
JOAB, I
中科院分区：
文献类型：
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作者：
MARTELRENOIR, D;GRUNEWALD, V;JOAB, I
我们最近发现，BZLF1（编码 Epstein-Barr 病毒 (EBV) ZEBRA 蛋白的基因）在所有八个研究的鼻咽癌 (NPC) 标本中都有表达。我们在此介绍了在相同的八个 NPC 活检中 EBV 裂解周期基因表达的研究，以确定 ZEBRA 反式激活子的产生是否可以导致完整的生产周期。肿瘤病变表现出多种不同的有限裂解基因表达模式。在八分之三的肿瘤中，无法检测到 BRLF1 和 BMLF1 表达。除此之外，BMLF1 mRNA 在所有其他样本中均表达。三个样本也表达 BRLF1。两个样本不仅表现出BZLF1、BMLF1和BRLF1转录本，而且还表达编码膜蛋白gp220的晚期基因BLLF1。在8个NPC中均未检测到早期基因产物BBLF2。然而，在 8 个 NPC 活组织检查中的 7 个中发现了编码 LMP1 裂解性截短形式 (D1LMP) 的晚期基因的表达。因此，可以表明 EBV 失败的裂解周期发生在大多数研究的 NPC 中。
We recently showed that BZLF1, the gene encoding the Epstein-Barr virus (EBV) ZEBRA protein, was expressed in all eight nasopharyngeal carcinoma (NPC) specimens studied. We present here studies on the expression of EBV lytic cycle genes in the same eight NPC biopsies to determine if production of the ZEBRA transactivator could lead to a complete productive cycle. The tumour lesions exhibit a number of different patterns of limited lytic gene expression. In three out of eight tumours neither BRLF1 nor BMLF1 expression could be detected. Otherwise BMLF1 mRNA was expressed in all the other specimens. Three specimens also expressed BRLF1. Two specimens not only exhibited BZLF1, BMLF1 and BRLF1 transcripts, but also expressed the late gene BLLF1 which encodes the membrane protein gp220. The early gene product BBLF2 could not be detected in any of the eight NPC. However, expression of the late gene encoding the lytic truncated form of LMP1 (D1LMP) was found in seven of the eight NPC biopsies. Thus, it could be suggested that the EBV abortive lytic cycle occurred in most of the NPC studied.