CgDREBa transgenic chrysanthemum confers drought and salinity tolerance
CgDREBa transgenic chrysanthemum confers drought and salinity tolerance
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CgDREBa 转基因菊花赋予干旱和盐度耐受性
DOI：
10.1016/j.envexpbot.2011.06.007
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发表时间：
2011-12-01
影响因子：
5.7
通讯作者：
Fang, Weimin
中科院分区：
文献类型：
--
作者：
Chen, Sumei;Cui, Xinli;Fang, Weimin
对匍匐地被菊品种‘雨花寻张’的耐旱性和耐盐性进行了比较，比较了其衍生的2个转基因品系的耐旱性和耐盐性，该品系携带菊花干旱响应元件结合因子（DREB） CgDREBa。CgDREBa的过表达赋予了对这两种胁迫的一定程度的耐受性。转基因品系表现出更高的成活率，并能在逆境中更好地保持鲜重。转基因植株叶片中超氧化物歧化酶、过氧化物酶活性和脯氨酸含量均高于转基因植株，但丙二醛积累较少。CgDREBa似乎是氧化和渗透稳态转导途径中基因的转录激活因子，代表了一个有希望的生物技术方法来提高植物的非生物胁迫耐受性水平。(C) 2011 Elsevier B.V.版权所有
The drought and salinity tolerances of a creeping ground-cover chrysanthemum variety 'Yuhuaxunzhang' were compared to the performance of its two derived transgenic lines carrying a drought-responsive element binding (DREB) factor from chrysanthemum designated as CgDREBa. The over-expression of CgDREBa conferred a measure of tolerance to both stresses. The transgenic lines showed a higher survival rate and were better able to retain fresh weight in the presence of stress. Activities of superoxide dismutase and peroxidase and the proline content were all higher in the leaves of the transgenic plants after a prolonged period of stress, but they accumulated less malondialdehyde. CgDREBa appears to function as a transcription activator of genes within the oxidative and osmotic homeostasis transduction pathways, and represents a promising candidate for a biotechnological approach to improve the level of abiotic stress tolerance in plants. (C) 2011 Elsevier B.V. All rights reserved.