COEXPRESSION OF DOPAMINE TRANSPORTER MESSENGER-RNA AND TYROSINE-HYDROXYLASE MESSENGER-RNA IN VENTRAL MESENCEPHALIC NEURONS
COEXPRESSION OF DOPAMINE TRANSPORTER MESSENGER-RNA AND TYROSINE-HYDROXYLASE MESSENGER-RNA IN VENTRAL MESENCEPHALIC NEURONS
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DOI：
10.1016/0169-328x(93)90059-x
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发表时间：
1993-12-01
期刊：
影响因子：
--
通讯作者：
EMSON, PC
中科院分区：
文献类型：
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作者：
AUGOOD, SJ;WESTMORE, K;EMSON, PC
放射性原位杂交用于绘制成年大鼠中枢神经系统中含有多巴胺 (DA) 转运蛋白 mRNA 的细胞的细胞定位。将含有 DA 转运蛋白 mRNA 的细胞的分布与经过处理的相邻切片进行比较，以可视化酪氨酸羟化酶 (TH) mRNA（含有儿茶酚胺的神经元的标记物）。使用碱性磷酸酶标记探针可视化，在下丘脑、中脑和脑桥中检测到含有 TH mRNA 的细胞；在黑质、腹侧被盖区和蓝斑中检测到最强的杂交信号。含DA转运蛋白mRNA的细胞分布较为受限；仅在黑质致密部和腹侧被盖区检测到强信号。在蓝斑中未检测到杂交信号。通过同时将中脑组织与碱性磷酸酶标记的 TH 探针和 S-35 标记的 DA 转运蛋白探针杂交，我们能够证明 DA 转运蛋白和 TH mRNA 由黑质和腹侧被盖区的相同细胞表达。含有 DA 转运蛋白 mRNA 的细胞的解剖定位受到限制，并且在蓝斑和其他肾上腺素能和去甲肾上腺素能细胞群中缺乏表达，证实了 DA 转运蛋白是大鼠脑中含有 DA 的神经细胞的突触前标记。
Radioactive in situ hybridization was used to map the cellular localization of dopamine (DA) transporter mRNA-containing cells in the adult rat central nervous system. The distribution of DA transporter mRNA-containing cells was compared to adjacent sections processed to visualize tyrosine hydroxylase (TH) mRNA, a marker of catecholamine containing neurones. TH mRNA-containing cells, visualized using an alkaline phosphatase labelled probe, were detected in the hypothalamus, midbrain and pons; the strongest hybridization signals being detected in the substantia nigra, ventral tegmental area and locus coeruleus. The distribution of DA transporter mRNA-containing cells was more restricted; a strong signal being detected in the substantia nigra pars compacta and ventral tegmental area only. No hybridization signal was detected in the locus coeruleus. By simultaneously hybridizing mesencephalic tissue with both the alkaline phosphatase-labelled TH probe and the S-35-labelled DA transporter probe we were able to demonstrate that both DA transporter and TH mRNAs are expressed by the same cells in the substantia nigra and ventral tegmental area. The restricted anatomical localization of DA transporter mRNA-containing cells and the lack of expression in the locus coeruleus and other adrenergic and noradrenergic cell groups confirms the DA transporter as a presynaptic marker of DA containing nerve cells in the rat brain.