A line scanning confocal fluorescent microscope using a CMOS rolling shutter as an adjustable aperture
A line scanning confocal fluorescent microscope using a CMOS rolling shutter as an adjustable aperture
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DOI：
10.1111/j.1365-2818.2012.03642.x
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发表时间：
2012-09-01
影响因子：
2
通讯作者：
Campion, M.
中科院分区：
文献类型：
--
作者：
Mei, E.;Fomitchov, P. A.;Campion, M.
传统的共焦显微镜使用物理孔径屏障来防止离焦光到达检测器。传统孔径的物理性质限制了对系统共焦的控制。我们描述了一种新型线扫描共焦显微镜，通过在 CMOS 相机上采用软件控制的卷帘快门，消除了对物理光圈的需求。通过滚动快门的同步运动和在样品上扫描的激光线来获得共焦图像。该显微镜的共焦分辨率可实时调节，并通过改变卷帘快门宽度为每个荧光通道独立建立。该技术已在 GE Healthcare 的 IN Cell Analyzer 6000 系统中实施。
Traditional confocal microscopy uses a physical aperture barrier to prevent out-of-focus light from reaching the detector. The physical nature of a conventional aperture limits control over the system confocality. We describe a new line scanning confocal microscope that eliminates a need for a physical aperture by employing a software-controllable rolling shutter on a CMOS camera. A confocal image is obtained by synchronizing motion of the rolling shutter and the laser line scanning over a sample. Confocal resolution of this microscope is adjustable in real time and independently established for each fluorescence channel by changing the rolling shutter width. This technology has been implemented in the IN Cell Analyzer 6000 system by GE Healthcare.