Some Characteristics of Movement of Indoleacetic Acid in Coleoptiles of Avena. I. Uptake, Destruction, Immobilization, & Distribution of IAA During Basipetal Translocation.
Some Characteristics of Movement of Indoleacetic Acid in Coleoptiles of Avena. I. Uptake, Destruction, Immobilization, & Distribution of IAA During Basipetal Translocation.
复制标题
燕麦胚芽鞘中吲哚乙酸运动的一些特征。
DOI：
10.1104/pp.37.4.492
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发表时间：
1962
期刊：
影响因子：
7.4
通讯作者：
K. Thimann
中科院分区：
文献类型：
--
作者：
Mary Helen;M. Goldsmith;K. Thimann
吲哚乙酸（Indoleacetic acid，IAA）是一种天然存在的植物生长素，控制着许多高等植物的正常营养生长。在许多植物中，芽的生长素在顶端或顶端附近产生（8，12，32），并且必须从产生部位转移到影响芽生长和分化的许多部位。植物生长素的易位，像其他有机分子，不能归因于扩散或纯粹的物理过程;它需要活细胞的存在（28，29，23）。生长素的移动与其他有机物质的转移不同，因为它主要发生在从茎尖向基部的转移。这不仅仅是生长素生产在顶点的反映。在燕麦胚芽鞘中，温特表明，即使生长素是在一个部分的基部提供的，它也不能在顶端收集（31）。自从Went（31）和货车der Weij（28，29）的最初工作以来，已经获得了关于植物组织转运天然生长素的能力（13）和各种物质对转运的抑制（例如5，16，17，18）的信息。然而，这些研究一直受到一些生物测定法的敏感性和适应性的限制。结论必须从生长素活性的量中得出，生长素活性出现在IAA来源的切片的另一端。这个实验系统是一个复杂的系统，人们想知道生长素从来源的吸收、破坏和在组织内的保留如何影响在切片基部回收的生长素的量。只有随着放射性标记IAA的出现，这种类型的定量研究才变得可行。这里提出的信息IAA的正常向基运输的切除部分提供了进一步研究的基础。例如，改变氧张力的影响和向顶运动的特征，非极性运动已经被研究过，并将在随后的论文中描述。
Indoleacetic acid (IAA) appears to be the major naturally occurring auxin controlling normal vegetative growth of a wide variety of higher plants. In many plants the auxin of the shoot is produced at or near the apex (8, 12, 32) and must be translocated from the site of production to the many sites where it influences growth and differentiation in the shoot. The translocation of auxin, like that of other organic molecules, can not be attributed to diffusion or purely physical processes; it requires the presence of living cells (28, 29, 23). The movement of auxin differs from the translocation of other organic substances in that it occurs predominantly from the apex toward the base of the shoot. This is not just-a reflection of auxin production at the apex. In Avena coleoptiles, Went showed that even when auxin is supplied at the base of a section it can not be collected at the apex (31). Since the initial work of Went (31) and van der Weij (28, 29), information has been obtained on the ability of plant tissues to translocate natural auxins (13) and on the inhibition of translocation by various substances (e.g. 5,16,17, 18). These studies, however, have been limited by the sensitivity and adaptability of some bioassay for detecting the auxin. Conclusions had to be drawn from the amount of auxin activity appearing at the opposite end of a section from a source of IAA. This experimental system is a complex one, and one would like to know how the uptake from the source, the destruction, and the retention of auxin within the tissue itself influence the amount of auxin recovered at the base of a section. Only with the advent of radioactively labeled IAA has this type of quantitative study become feasible. The information presented here on the normal basipetal transport of IAA by excised sections provides a basis for further studies. For example, the effect of altered oxygen tensions and the characteristics of an acropetal, apolar movement have already been examined and will be described in subsequent papers.