Testing of A size criterion for DNA–hydrocarbon binding
Testing of A size criterion for DNA–hydrocarbon binding
复制标题
DNA-碳氢化合物结合的 A 尺寸标准测试
作者：
Morrie Craig;I. Isenberg
DNA-烃结合的嵌入模型似乎是合理的，但仅依赖于间接证据。为了检验该模型，已提出了一个尺寸标准的约束。大小标准是基于疏水力在烃与DNA的结合中起主要作用的假设。它指出，小到足以嵌入DNA并被很好地保护而不与介质接触的碳氢化合物将被发现与DNA结合;那些太大的碳氢化合物则不会。我们报告的结果结合十四个多环芳烃的DNA。基于尺寸标准的预测被认为是有效的，在所有情况下测试。
The intercalation model of DNA–hydrocarbon binding appears reasonable, but rests on indirect evidence only. To test the model, a size criterion for binding has been proposed. The size criterion is based on the assumption that hydrophobic forces play a major role in the binding of hydrocarbons to DNA. It states that hydrocarbons which are small enough to intercalate into DNA and be well protected from contact with the medium, will be found to bind to DNA; those that are too large will not. We report results on the binding of fourteen polycyclic aromatic hydrocarbons to DNA. Predictions based on the size criterion were found to be valid in all cases tested.