Induction of endosomal/lysosomal pathways in differentiating osteoblasts as revealed by combined proteomic and transcriptomic analyses
Induction of endosomal/lysosomal pathways in differentiating osteoblasts as revealed by combined proteomic and transcriptomic analyses
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DOI：
10.1016/j.febslet.2010.07.055
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发表时间：
2010-09
期刊：
影响因子：
3.5
通讯作者：
Takako Taniguchi;S. Kido;E. Yamauchi;M. Abe;Toshio Matsumoto;H. Taniguchi
中科院分区：
文献类型：
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作者：
Takako Taniguchi;S. Kido;E. Yamauchi;M. Abe;Toshio Matsumoto;H. Taniguchi
我们利用体外分化模型，通过定量差异蛋白质组学和转录组学分析，分析了与成骨细胞分化相关的蛋白质组变化。在蛋白质水平上发现69个蛋白质上调(2倍)，18个下调(0.5倍)。采用实时定量聚合酶链式反应和聚类分析相结合的方法对这些蛋白的基因表达水平进行分析。蛋白和mRNA水平增加的最显著的簇包含内体和溶酶体蛋白，表明成骨细胞内降解的内体/溶酶体途径被强烈诱导。因此，成骨细胞不仅参与细胞外基质蛋白的合成，而且还参与细胞外基质蛋白的周转。
We have analyzed proteome changes associated with bone-forming osteoblast differentiation by quantitative differential proteomic and transcriptomic analyses using in vitro differentiation model. Sixty nine proteins were found up-regulated (>2-fold) and 18 were down-regulated (<0.5-fold) at protein level. The mRNA levels of these proteins were then analyzed by quantitative real-time PCR combined with clustering analysis. The most prominent cluster with increased protein and mRNA levels contains endosomal and lysosomal proteins, demonstrating the drastic induction of degradative endosomal/lysosomal pathways in osteoblasts. Osteoblasts, therefore, are involved not only in the synthesis but also in the turnover of the extracellular matrix proteins such as collagens.