Left ventricular haemodynamics in murine viral myocarditis
Left ventricular haemodynamics in murine viral myocarditis
复制标题
DOI：
10.1093/eurheartj/16.suppl_o.28
复制
发表时间：
1995-12-01
影响因子：
39.3
通讯作者：
Maisch, B
中科院分区：
文献类型：
--
作者：
Herzum, M;Mahr, P;Maisch, B
在易感的DBA/2中，感染柯萨奇病毒B3的小鼠会导致严重的心脏炎症和坏死性病变。心脏中复制病毒的峰值浓度和最大的心脏炎症存在时间上的差异。这项研究的目的是，第一，确定柯萨奇病毒BS诱导的小鼠心肌炎是否发生血流动力学变化，第二，确定这些变化可能明显的时间框架。经左心室穿刺术，可在感染后数天测得左心功能参数--压力和一阶导数dp/dt。心脏横切面的苏木精-伊红染色显示了心脏炎症损害的过程；空斑形成试验评估了心脏中的病毒滴度。复制病毒在心脏内的浓度在感染后第3天达到高峰，心脏炎症病变在感染后第7天达到高峰。左心功能基本保持到感染后第5天，然后显著下降到IO天。这项研究表明，无论是病毒介导的肌纤维累积破坏，还是病毒引发的对心脏细胞的免疫反应，都会导致左心功能受损。
In susceptible DBA/2, mice infection with coxsackievirus B3 leads to severe inflammatory and necrotic lesions in the heart. There is a temporal discrepancy of peak concentrations of replicative virus in the heart and maximal cardiac inflammation. Aims of this study were, first, to determine whether haemodynamic changes occur in coxsackievirus BS-induced murine myocarditis and, second, the time frame in which those alterations may be apparent. By puncture of the left ventricle, pressures and the first derivative dp/dt as parameters of left ventricular function could be obtained on several days of the infection. Haematoxylin-eosin stains of cross-sections of the heart showed the course of inflammatory lesions in the heart; a plaque forming assay assessed virus titres in the heart. Cardiac concentrations of replicative replicative virus peaked on day 3, inflammatory lesions in the heart were maximal on day 7. Left ventricular function was almost preserved until day 5 of the infection, then dropped significantly until day IO. The study suggests that either a cumulative virus-mediated destruction of the myofibres or virally triggered immune reactions to heart cells lead to impairment of left ventricular function.