Synthesis of polyester by means of genetic code reprogramming
Synthesis of polyester by means of genetic code reprogramming
复制标题
DOI：
10.1016/j.chembiol.2007.10.015
复制
发表时间：
2007-12-01
影响因子：
--
通讯作者：
Suga, Hiroaki
中科院分区：
文献类型：
--
作者：
Ohta, Atsushi;Murakami, Hiroshi;Suga, Hiroaki
在这里，我们报告的核糖体聚合的α-羟基酸的遗传密码重编程的手段。flexizyme系统是一种基于核酶的tRNA酰化工具，用于将单个密码子重新分配给七种类型的α-羟基酸，然后使用重建的无细胞翻译系统在重编程遗传密码的控制下合成聚酯。聚酯片段的序列和长度由mRNA模板指定，表明聚酯的高保真核糖体表达是可能的。这项工作打开了一扇门的mRNA指导合成骨架改变的生物聚合物。
Here we report the ribosomal polymerization of alpha-hydroxy acids by means of genetic code reprogramming. The flexizyme system, a ribozyme-based tRNA acylation tool, was used to reassign individual codons to seven types of a-hydroxy acids, and then polyesters were synthesized under controls of the reprogrammed genetic code using a reconstituted cell-free translation system. The sequence and length of the polyester segments were specified by the mRNA template, indicating that high-fidelity ribosome expression of polyesters was possible. This work opens a door for the mRNA-directed synthesis of backbone-altered biopolymers.